Luer fittings

Connect a vacuum manifold to a vacuum source not to exceed 20 in Hg, and place a 1-L waste liquid trap between the manifold and the vacuum source. To use the manifold, remove the cover, and place a Luer hub solvent guide needle on the male Luer fitting of each flow control valve. Remove the collecting vessel rack, and replace the cover. Close all control valves on the manifold cover. 

SPE disc technology through rigid disc and membrane disc formats provides a way around the above-listed limitations of packed-bed SPE columns. SPE discs closely resemble membrane filters they are flat, usually 1 mm or less in thickness, with diameters varying in size. Some disks are sold loose, and users must install them in a filter holder. Others are sold preloaded in disposable holders with Luer fittings (82). 

FCA and FLA FCA is a suspension of killed pertussis m oil This should be shaken well before use FIA is just oil Freund s adjuvants should be mixed with the aqueous immunogen solution/suspension at a ratio between 1 2 and 2-1 and mixed until set. This is most easily achieved by passage back and forth between two glass syringes connected by a three-way luer fitting. After a while (1—5 min) the mixture should become noticeably stiffer, and may then be used. 

When joining tubes together with a luer lock connection, care must be taken that the tubes have a degree of twist that is always ensunng that the luer fittings remain locked. They can work loose because of the movement in the apparatus generated by the peristaltic pump... 

Membrane filters composed of cellulose esters can be used to collect samples. The filters, about 25 pm thick, are supplied in matched pairs to compensate for their absorption in a double-beam instrument. The filter is placed in a specially designed metal and PTFE holder. The PTFE inlet section reduces condensation of the effluent before it reaches the filter and the metal outlet section allows the filter to be cooled. The filt hold is connected to the outlet of tile diromatograph by a Luer fitting just before the sample emerges. When the sample has been collected, the filter is removed and placed in the spectrophotomet. The method is simple and easy to use and is capable of giving extremely good results. 

The sample is introduced into the system via an injection valve, but this - a much simpler and less expensive valve than that found in an hplc 75tem. We use a Rheodyne type 50 Teflon rotary valve, which will take a 3w rate of lOOml/min (Fig. 11.24). The pump and the column are -lanected to the red and white connectors respectively, whilst a sample load 33p (see below) is fitted to the black and yellow connectors. A Luer fitting. 3 attached to the blue connector, which is where the sample is introduced, ->ing a syringe. The green connector is a vent and should be positioned so jiat effluent from it can be collected in a beaker or flask. 

Filter Stick. A typical filter stick for solution filtration is shown in Figure 4. A section of teflon tubing (2-3 ft) is pushed over the Luer fitting of an observation port (Popper Sons), and conventional filter paper is then attached to the end of the observation port as shown. Half inch teflon sealing tape (Aldrich - this may also be available from a local plumbers supplier as pipe dope) is first wrapped around the base of the observation port, filter paper is then wrapped around the outside of the teflon tape, and one or two loops of nichrome wire (20 gauge, 32 thou) are used to secure the filter paper in place. The teflon tape provides a bed into which the wire can bite and seal the system. Any filter paper may be used, but we have found water resistant fine filter papers such as Whatman s 50 to be most satisfactory, except for gummy solids which may require a faster paper. 

Figure 4. Conversion of an observation port into a filter stick. The luggs can be sawed off the Luer fitting on the observation tube to make a more streamlined apparatus. A second loop of nichrome wire may be convenient to prevent leakage.

Upchurch Scientific produce Nanoport , which is a line of assemblies that bond to chip surfaces and provide a fluid connection via PEEK (polyetheretherketone) tubing. Other interconnects such as Luer fittings and connectors for HPLC may also be used. Sandia National Laboratories has also developed a line of interconnects and ferrules specifically for microfluidic devices. 

Figure 2 Examples of liquid cells for mId-Infrared transmission measurements (A) sealed vacuum tight liquid cell with an amalgam spacer for FT-IR, (B) exploded view of a liquid cell design with a rectangular aperture for use with a dispersive infrared spectrometer, and (C) semipermanent (demountable) liquid cell for FT-IR with Teflon spacer, and fitted with a Luer fitting and Teflon plugs.

The spike is used to connect the system to an infusion bag (or a bottle). The drip chamber is used in combination with the roller clamp to control the flow rate. The drip chambers of the administration systems are equipped with 15 pm filters which ensure particle removal. The system is connected to an access point (e.g. venous cannula) with a Luer fitting. Often access points are available to connect a second infusion. 

Fig. 13.9 Administration set for infusion by gravity (1. Spike 2. Drip chamber 3. Access point for the connectirai of another infusion bag 4. Roller clamp 5. Luer fitting). Source Recepteeikunde 2009, KNMP...

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