Luer lock

Fig. 11 Experimental set-up for small-scale microwave SPPS of /S-peptides (SPE = solid-phase extraction). 1 Pasteur pipet for N2 agitation 2 10 mL glass vial 3 4mL solid-phase extraction tube 4 DMF 5 coupling solution 6 resin 7 polyethylene frit 8 Luer-lock cap...

Fig. 12 Expandable microwave vessel. Components I Luer-lock cap 2 piston 3 hollow bore 4 reaction chamber 5 frit 6 product outlet 7 Luer-lock cap...

Pre-column HPLC Alter, ulAa-low dead volume, 0.5- im frit (Upchurch, A-318) Cartridge adapters, SPE (University Research Glass, Cat. No. URG-2440-SPECA) Stopcocks (plasAc), Luer Lock (Varian, 1213-1005)... 

HPLC, ConstaMetric 3500 MS and ConstaMetric 3200 MS Mass spectrometer, TSQ 7000 with atmospheric pressure ionization (API) electrospray interface Robotcoupe, Model RSI 25 Syringes, Luer lock, 10-mL... 

NEEDLE HYPOC12GP 22GA 1.50" LG LUER LOCK PLASTIC HUB STER 100S 6515011727650 PG 4.36 ... 

NEEDLE HYPO C13A GP 1SGA 1.433-1,562" LG LUER LOCK REG STER 100S 6515007542834 PG 6.63 ... 

NEEDLE HYPO C13AGP20GA 1.433-1,562 LG LUER LOCK REG STER 10QS 6515007542836 PG 6.17 ... 

NEEDLE HYPO C13A GP25GA. 57S-.672" LG LUER LOCK STER DISP 100S 6515006555751 PG 3.75 ... 

NEEDLE HYPO C20A SPINAL 22GA 3.375-3,625" LG LUER LOCK REG 25S 6515011039996 PG 30.36 ... 

NEEDLE HYPO C2QA SPINAL 25GAX3.375-3.625 LG LUER LOCK HUB REG25S 6515011039995 PG 28,69 ... 

SYRINGE HYPO 10/12ML CAPACITY LUER LOCK CONCENTRICT1P DISP 100S 6515011680108 PG 114.75 ... 

SYRINGE HYPODERMIC GP 30ML CAPACITY LUER LOCK TIP STER DISP 25S 6515011656742 PG 14.47 ... 

SYRINGE HYPODERMIC GP3ML CAP LUER LOCK TIP PLAS STER DISP 100S 6515004627348 PG 6.66 ... 

We point out here that the colloid prepared by these methods is very clean, because the carrier gas used is usually high-purity grade at six-nine, the chamber is once evacuated to depress the extent of contaminating oxygen and moisture, and the liquids themselves are always purified by sublimation process except for the solution trap method. To transfer the colloidal suspension after preparation, a specially designed stock bottle with a Luer-lock syringe is normally used in order to enable the operations under Ar flow to avoid unexpected air contamination. Therefore, we can carry the suspension liquid away from the production chamber without exposure to air, which means that the surface of colloidal metal is very clean if it does not react with suspension liquids. 

Ass a stainless steel cannula was not available, the checkers made a minori modification in the operation without any trouble. They transferred fthe supernatant pentane and the solution of the sodium enolate using a Luer-lock hypodermic syringe with a stainless steel needle, prefluslbed with nitrogen, and the apparatus was swept with nitrogen during this operation. 

The synthesis is performed in standard Schlenk equipment4 under a carbon monoxide atmosphere, if not otherwise stated. Transfer of liquid is carried out with hypodermic glass syringes equipped with Luer lock valves. All solvents are laboratory grade, distilled and stored under inert atmosphere. 

Two glass luer lock syringes 2 mL is the best size. 

Luer lock connectors double female (cat. no. 892.00, Vygon Ltd.). 

Luer lock male adapter (MTLI250, Value Plastics Inc, Fort Collins, CO). 

Product access port connectors (2/cartridge) Push a male/female adapter R90 into the barb hose part of the dialysate hose connector and reinforce with 2 cm of tubing (see Fig. 2). Protect adapter end with a male luer lock cap R96. 

Continue to pump for about 10 s after all of the medium has passed through to confirm integrity of the filter by disconnecting the pump tubing from the filter at the luer lock and releasing the pressure If the pressure is not considerable, then the integrity of the filter is m doubt, and the media must be refiltered... 

Many types of in-line filter are available, but few have a luer lock fitting for both the inlet and outlet. Wherever possible, the use of luer lock fittings is recommended for ease of manipulation and greatest adaptability... 

When joining tubes together with a luer lock connection, care must be taken that the tubes have a degree of twist that is always ensunng that the luer fittings remain locked. They can work loose because of the movement in the apparatus generated by the peristaltic pump... 

Figure 7.4 Microwave vessel components (left) (1) reaction chamber (2) Frit (3) Luer lock (4) product outlet (5) piston (6) Luer lock (7) hollow bore (8) seal. Single microwave vessel during reaction (right) (1) central bore closed (2) cover plate (3) reaction components (4) product outlet closed (5) supporting plate. (Reproduced with permission from Ref. 21).

The hybridomas and secreted antibody are retained in the production module (Fig. 2). Depending on the cell line, cell densities can reach upwards of 1 x 107 cells/mL. The MiniPERM bioreactor is rotated on a universal turning device that allows four MiniPERM bioreactors to be run simultaneously for larger production runs of one hybridoma or production of different hybridomas. This motion speeds the distribution of nutrients to the cells and facilitates the removal of metabolic waste products and C02, processes that rely on passive diffusion in static bioreactors. Sample collection and harvesting is via Luer-Lock connections on the production module. The MiniPERM is a continuously fed culture system that allows cultures to be maintained for several weeks. 

After sampling and harvesting the Luer-Locks must be wiped down with 70% ethanol to prevent infection being introduced to the production module see Note 20). Periodical measurement of cell density, viability, and antibody production during the course of the production run will help in the planning of future production runs. 

Replacement sterile caps for the nutrient module Luer-Locks can be used to reduce the chances of contamination. 

Spare Luer-Lock screw caps for production modules six pieces, single packed, sterile (cat. no. IV-76077027 Vivascience). 

Draw the suspension slowly up into a 50-mL hypodermic syringe (see Note 13). Unscrew the two Luer-Locks on the assembled MiniPERM and inject the cell suspension carefully into the production module while the module is slowly rotated (see Note 14). This ensures that displaced air is expelled through the open Luer-Lock. 

Replace both Luer-Locks then add 400 mL of nutrient medium to the nutrient module. Close the cap and place the MiniPERM module inside an incubator on the Universal turning device set at 5 rpm (see Note 15). 

Pressure within the module can cause the cell-culture medium to spurt out when the Luer-Locks are opened. If the rubber membrane... 

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