Lissamine rhodamine B sulfonyl

Remove unreacted NHS-rhodamine and reaction by-products by gel filtration or dialysis. Lissamine Rhodamine B Sulfonyl Chloride... 

Figure 9.16 Lissamine rhodamine B sulfonyl chloride reacts with amine-containing molecules to produce stable sulfonamide bonds.

Lissamine rhodamine B sulfonyl chloride is relatively insoluble in water, but may be dissolved in DMF prior to the addition of a small aliquot to an aqueous reaction. Do not dissolve in DMSO, as sulfonyl chlorides will readily react with this solvent (Boyle, 1966). The compound has a maximal absorptivity at 556 nm with an extremely high extinction coefficient of up to 93,000M em-1 (in methanol) in highly purified form. Its emission maximum occurs at 576 nm, emitting red luminescence. 

Lissamine rhodamine B sulfonyl chloride has been used in numerous applications, including multiple-labeling techniques in microscopy (Wessendorf, 1990), for confocal microscopy... 

The following protocol is a general guide for labeling biological macromolecules with Lissamine rhodamine B sulfonyl chloride. Optimization of the fluorophore incorporation level (F/P ratio) may have to be done for specific labeling experiments. 

Dissolve Lissamine rhodamine B sulfonyl chloride (Invitrogen) in DMF at a concentration of l-2mg/ml. Protect from light and use immediately. 

Lissamine rhodamine B sulfonyl hydrazine is a hydrazide derivative of sulforhodamine B that can spontaneously react with aldehyde- or ketone-containing molecules to form a covalent,... 

Lissamine rhodamine B sulfonyl hydrazine can react with aldehyde groups to form hydrazone linkages. 

Lissamine rhodamine B sulfonyl hydrazine is soluble in DMF. The reagent may be dissolved in this solvent as a concentrated stock solution before adding a small aliquot to an aqueous reaction medium. The compound itself and all solutions made with it should be protected from light to avoid decomposition of its fluorescent properties. 

Modification with Lissamine Rhodamine B Sulfonyl Chloride... 

Estradiol 5-Dimethylaminonaphthalene-l -sulfonyl chloride Lissamine rhodamine B sulfonyl chloride 87... 

Lissamin rhodamine B sulfonyl chloride chemiluminescence reagent, 5 852-853 Lister, Joseph, 11 7 Literature research, in fine chemical... 

Lissamine rhodamine B sulfonyl hydrazine is a hydrazide derivative of sulforhodamine B that can spontaneously react with aldehyde- or ketone-containing molecules to form a covalent, hydrazone linkage (Fig. 217). It also can be used to label cytosine residues in DNA or RNA by use of the bisulfite activation procedure (Chapter 17, Section 2.1). The resulting fluorescent derivative exhibits an excitation maximum at a wavelength of 556 nm and a maximal emission wavelength of 580 nm when dissolved in methanol. In... 

Generalized protocols for the use of hydrazine-probes reactive toward aldehyde residues can be found in Section 1.1. These procedures are directed at the labeling of cell surface glycoproteins or glycoproteins in solution. Substitution of Lissamine rhodamine B sulfonyl hydrazine for the fluorescein-5 -thiosemicarbazide reagent described in that section can be done without difficulty. Some optimization may be... 

Grammer et al. [120] studied collagen shield with dissolution times of 12 h, that were presoaked with either hydrophilic or lipophilic fluorophore (4,5-carboxyfluorescein and jV-[Lissamine rhodamine B sulfonyl]-diacyl-phosphatidylethanolamine, respectively), in a solution or unilamellar liposome suspension with different surface charges and bilayer fluidity. For the hydrophilic fluorophore, two to seven times higher concentrations were achieved in the collagen shield by immersion in aqueous solution than immersion in the liposome suspensions. 

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