Liquid water medium glucose

Liquid Culture Medium Dissolve 15 g of peptonized milk, 5 g of water-soluble yeast extract, 10 g of anhydrous glucose, and 2 g of anhydrous potassium dihydrogen phosphate in about 600 mL of water. Add 100 mL of filtered tomato juice (filtered through Whatman No. 1 filter paper, or equivalent), and adjust to pH 6.5 by the dropwise addition of 1.0 N sodium hydroxide. Add, with mixing, 10 mL of the Polysorbate 80 Solution. Dilute with water to a final volume of 1000 mL. Add 10-mL portions of this Liquid Culture Medium to test tubes, cover to prevent contamination, and sterilize by heating in an autoclave at 121° for 15 min. Cool the tubes rapidly to keep color formation to a minimum, and store at 10° in the dark. 

The California strain of giganteum (ATCC 52675) maintained as previously described (Kerwin and Washino, 1986a) was used in these investigations. Oosporogenesls was Induced in liquid shake culture using 1 liter of medium in 2800 ml fernbach flasks. Media consisted of (gram/llter of deionized water) 1 glucose, 1 yeast extract, 1 hydrolyzed lactalbumln,... 

YE liquid medium. 0.5% yeast extract, 2% glucose, 50 pg/ml adenine. To make 1 1 of this medium, these materials are added to 1 1 of water, dissolved well, and then autoclaved (120°C, 10-15 min). The concentration of glucose is often increased to 3%. 

The brewer next prepares the wort, the nutrient medium required for fermentation by yeast cells. The malt is mixed with water and then mashed or crushed. This allows the enzymes formed in the malting process to act on the cereal polysaccharides to form maltose, glucose, and other simple sugars, which are soluble in the aqueous medium. The remaining cell matter is then separated, and the liquid wort is boiled with hops to give flavor. The wort is cooled and then aerated. 

Activity of calcium phosphate dissolution by the tested bacterial strain was evaluated on liquid glucose-asparagine medium with phosphates [3]. Water-soluble phosphates wee determined according to Taussky and Shore, and at the end of experiment potentiometrically [4]. Amount of indolyl-3-acetic acid (lAA) produced by rhizobacteria was estimated by colorimetric method [5]. 

Ionic liquids (IL) can be used as solvents for nucleophilic substitution reactions of alkyl halides or tosylates with NaN3. ° The authors studied three ionic liquids (84 and 85), [bmim][PF6], [bmim][N(Tf)2]> [hpyr][N(Tf)2] (where bmim = l-butyl-3-methyl-imidazo-lium, hpyr = 1-hexylpyridinium, PFg = hexafluorophosphate, N(Tf)2 = bis(trifluoromethy lsulfonyl)imide). It was observed that nucleofugacity scales for this reaction are similar to those reported for the same process in cyclohexane. It was also observed that elimination reaction does not compete with substitution even in cases with sterically hindered substrates such as the triflate ester of diacetone-D-glucose 81. The nucleophilic displacement on n-octyl mesylate (86) with potassium azide in a biphase system of supercritical carbon dioxide (SCCO2) and water, in the presence of catalyst Bu4PBr is also an adequate medium for the synthesis of the corresponding azide 87 ° (Scheme 3.11). 

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