Lipoxygenase regulating

Romano M, Catalano A, Nutini M, D Urbano E, Crescenzi C, Claria J, Libner R, Davi G, Procopio A. 5-lipoxygenase regulates malignant mesothelial cell survival involvement of vascular endothelial growth factor. FASEB J. 15 (2001) 2326-2336. 

It is possible that dietary flavonoids participate in the regulation of cellular function independent of their antioxidant properties. Other non-antioxidant direct effects reported include inhibition of prooxidant enzymes (xanthine oxidase, NAD(P)H oxidase, lipoxygenases), induction of antioxidant enzymes (superoxide dismutase, gluthathione peroxidase, glutathione S-transferase), and inhibition of redox-sensitive transcription factors. 

Hatzelman, A. and Ullrich, V. (1987). Regulation of 5-lipoxygenase activity by the glutathione status in human polymorphonuclear leukocytes. Eur. J. Biochem. 169, 175-184. 

At the end of the reaction, hydroperoxide can be easily recovered in the aqueous phase (98-99%) after its separation from the organic phase and precipitation of the enzymes. The hydroperoxides obtained are highly reactive molecules [109]. They are intermediate compounds in the lipoxygenase pathway in plants, precursors for the synthesis of hydroxy-fatty acids (i.e., ( + )-coriolic acid [38,110], and regulators of the prostaglandins biosynthesis [111-113]. 

CysLTs) are proinflammatory molecules synthesized primarily by basophils, neutrophils, and mast cells and are potent mediators of airway inflammation and bronchoconstriction (77,78). There are two classifications of drugs in this category that can regulate the effects of CysLTs inhibitors of the 5-lipoxygenase enzyme, such as zileuton, and CysLT receptor antagonists, such as montelukast and zafirlukast. 

Brock TG. (2005) Regulating leukotriene synthesis the role of nuclear 5-lipoxygenase. J Cell Biochem. 96, 1203-1211. 

Royo, J., Vancanneyt, G., Perez, A. G., Sanz, C., Stormann, K., Rosahl, S., Sanchez-Serrano, J. J. (1996). Char-aeterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns. J. Biol. Chem., 271, 21012-21019. 

Uz T., Pesold C., Longone P., and Manev H. (1998). Aging-associated up-regulation of neuronal 5-lipoxygenase expression putative role in neuronal vulnerability. FASEB J. 12 439 449. 

Menard C., Valastro B., Martel M. A., Chartier T., Marineau A., Baudry M., and Massicotte G. (2005). AMPA receptor phosphorylation is selectively regulated by constitutive phospholipase A2 and 5-lipoxygenase activities. Hippocampus 15 370-380. 

Etingin OR, Hajjar DP. Evidence for cytokine regulation of cholesterol metabolism in herpesvirus-infected arterial cells by the lipoxygenase pathway. J Lipid Res 1990 31 299-305. 

Tang, S., Bhatia,B., Maldonado, C.J., et al., Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells, J. Biol. Chem., 211, 16189, 2002. 

The rate-limiting step in the biosynthesis of eicosanoids is the availabiUty of free precursor, unesterified AA (20 4, o)-6), for both cyclooxygenase (COX) and lipoxygenase en2ymes (13). The initial mobilization cellular AA (20 4, (0-6) is an essential step in the synthesis of eicosanoids (14, 15). Cellular AA is known to be exclusively associated with membrane phospholipids (16-18). It is also tigfrtly regulated through enzymes of the Lands cycle. The enzymes such as phospholipase Aj, arachidonoyl-CoA synthetase and lysophosphatidyl acyltransferases appear to be simultaneously active in order to maintain a steady turnover of AA (20 4, -6) (19). Platelets contain arachidonoyl CoA synthetase (20). 

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