Lipoteichoic acid biosynthesis

In another study, Boaretti et al, (75) have shown that daptomycin at the MIC in the presence of causes inhibition of protoplast regeneration in H. faedum, whereas vancomycin does not. They showed that daptomycin caused 82% inhibition of lipotei-choic acid biosynthesis in E. faedum protoplasts, 40% inhibition of lipid biosynthesis, and only 14, 17, and 23% inhibition of peptidoglycan, DNA, and protein biosynthesis, respectively. They demonstrated Ca -dependent tight binding of daptomycin to the cytoplasmic membrane. These data are consistent with the notion that lipoteichoic acid biosynthesis is the primary target of daptomycin at low concentrations. 

Liu F-L, Chuang C-Y, Tai Y-T et al (2012) Lipoteichoic acid induces surfactant protein-A biosynthesis in human alveolar type II epithelial cells through activating the MEK1 /2 -ERK1 /2 -NF -kB pathway. Respir Res 13 88... 

Glaser, L. Lindsay, B. (1974) The Synthesis of Lipoteichoic Acid Carrier , Biochemical and Biophysical Research Communications, 59, 1131-6 Gold, M.H. Hahn, H.J. (1976) Role of a Mannosyl Lipid Intermediate in the Synthesis of Neurospora crassa Glycoproteins, Biochemistry, 15,1808-14 Goldemberg, S.H. Marechal, L.R. (1963) Biosynthesis of Paramylon in Euglena gracilis, Biochimica et Biophysica Acta, 71, 743-4... 

Heaton MP, Neuhaus FC. Biosynthesis of D-alanyl-lipoteichoic acid Cloning, nucleotide sequence, and expression of the LoctobadDus casei gene for the D-alanine-activating enzyme. J Bacteriol 1992 174 4707-4717. 

Heaton, MP. Neuhaus, FC. Role of the Oalanyl carrier protein in the biosynthesis of [>alanyl-lipoteichoic acid.] Bacteriol 1994 176 681-690. 

Canepari et al. (74) have shown that at the MIC, daptomycin caused only partial blockage of DNA, RNA, protein, and peptidoglycan biosynthesis in . faecium. In contrast, it caused >50% inhibition of radiolabeled phosphate into teichoic acid and >90% inhibition of incorporation of radiolabeled glycerol into lipoteichoic acid in 20 min in both S. aureus and in . faecium. They also showed that daptomycin does not penetrate bacterial cells, but binds reversibly to cell walls and irreversibly to cell membrane in the... 

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