Liposome Chromatography

A method where phospholipids are entrapped in the pores of resin beads, in the forms of multilamellar vesicles, has been described [313-319,376]. In some ways, the idea is similar to that of IAM chromatography, even though the resin is modified differently. The retention indices correlate very well with the partition coefficients measured in liposome-water systems (described below). 

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Lundahl, P., Beigi, F. Immobilized liposome chromatography of drugs for model analysis of drug-membrane interactions. Adv. Drug Deliv. Rev. 1997, 23, 221-227. 

Immobilized liposome chromatography (ILC) is an (albeit related) alternative to IAM [96, 97]. Compounds with the same log P were shown to have very different degrees of membrane partitioning on ILC, depending on the charge of the compound [97]. 

ILC, immobilized liposome chromatography MEKC, micellar electrokinetic capillary chromatography. 

C Lagerquist, F Beigi, A Karlen, H Lennernas, P Lundahl. Effects of cholesterol and model transmembrane proteins on drug partitioning into lipid bilayers as analyzed by immobilized-liposome chromatography. J Pharm Pharmacol 53 1477-1487, 2001. 

X-Y Liu, Q Yang, N Kamo, J Miyake. Effect of liposome type and membrane fluidity on drug-membrane partitioning analyzed by immobilized liposome chromatography. J Chromatogr A 913 123-131, 2001. 

Y Zhang, S Aimoto, L Lu, Q Yang, P Lundahl. Immobilized liposome chromatography for analysis of interactions between lipid bilayers and peptides. Anal Biochem 229 291-298, 1995. 

P Lundahl, Q Yang. Liposome chromatography a new mode of separation using lipid bilayer. Protein Nucleic acid Enzyme (Tokyo) 35 1983-1998, 1990 (in Japanese). 

P Lundahl, Q Yang. Liposome chromatography liposomes immobilized in gel beads as a stationary phase for aqueous column chromatography. J Chromatogr 544 283-304, 1991. 

Falten et al. recently reported that phospholipid membrane vesicles can be con-stmcted on a filter scaffold without any organic solvent [57-60]. In this system, the phospholipid vesicle occupies the filter pores to form a permeation barrier. This is more relevant to the cellular membrane than PAMPA membranes with organic solvent. The membrane can be stored up to two weeks without significant change and is stable at pH 2-8. The Fa% predictability was compared with BM-PAMPA, DS-PAMPA, Caco-2 and immobilized liposome chromatography, resulting in promising predictability. 

The partition coefficients of triphenylalkylphosphonium homologs have been determined in gel bead-immobilized small or large unilamellar liposomes by chromatography [8]. It was claimed that the technique, immobilized liposome chromatography (ILC), is suitable for the determination of membrane partition coefficients of drugs. 

Chromatography also can be used to predict permeability. The most widely used methods are immobilized artificial membrane chromatography (lAM) and immobilized liposome chromatography (ILC). 

Lundahl P and Beigi F. Immobilized Liposome Chromatography of Drugs for Model Analysis of Drug-Membrane Interactions. Adv Drug Deliv Rev 1997 23 221-227. 

Yoshimoto M, and Kuboi R, Oxidative refolding of denatured/reduced lysozyme utilizing the chaperone-like function of liposomes and immobilized liposome chromatography, Biotechnol. Progr. 1999 15 480-487. 

Liposome chromatography has also recently been used as a tool for predicting permeability (Lundahl and Beigi 1997 Beigi et al. 1998). For most drugs tested, the method does not predict drug absorption better than other chromatographic methods, such as reversed-phase... 

Boija, E., Johansson, G. Interaction between model membranes and lignin-related compounds studied by immobilized liposome chromatography. Biochim. Biophys. Acta 1758, 620-626 (2006)... 

A comprehensive two-dimensional HPLC system, with an RP column as a primary column and an immobilized liposome chromatography (ELC) column as a secondary column, was developed for the screening and analysis of the membrane-permeable compounds in the traditional Chinese medicine... 

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