Immobilized liposome chromatography, ILC

Immobilized liposome chromatography (ILC) is an (albeit related) alternative to IAM [96, 97]. Compounds with the same log P were shown to have very different degrees of membrane partitioning on ILC, depending on the charge of the compound [97]. 

The partition coefficients of triphenylalkylphosphonium homologs have been determined in gel bead-immobilized small or large unilamellar liposomes by chromatography [8]. It was claimed that the technique, immobilized liposome chromatography (ILC), is suitable for the determination of membrane partition coefficients of drugs. 

Chromatography also can be used to predict permeability. The most widely used methods are immobilized artificial membrane chromatography (lAM) and immobilized liposome chromatography (ILC). 

ILC, immobilized liposome chromatography MEKC, micellar electrokinetic capillary chromatography. 

Compared with lAM, which uses a monolayer of phospholipid, the liposomal phospholipid bilayers in ILC have the advantage of closely resembling biologic membrane bilayers and constitute a 2-D fluid in which lipid molecules and other components are free to diffuse (10). With this technique, the phospholipid composition can be changed to mimic the membrane of interest. Membrane lipids extracted from human cells also can be used the technique then is called immobilized biomembrane chromatography (IBC) (11). 

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