Lipoproteins distribution

Rossignol DP, Wasan KM, Choo E, Yau E, Wong N, Rose 1, Moran 1, Lynn M. (2004) Safety, pharmacokinetics, pharmacodynamics and plasma lipoprotein distribution of eritoran (E5564) during continuous intravenous infusion into healthy volunteers. Antimicrob Agents Chemother 48 3233-3240. 

Phenotypes of abnormal lipoprotein distribution are described in this section. Drugs mentioned for use in these conditions are described in the following section on basic and clinical pharmacology. 

A method for determination of TBARS in the plasma of a 50 p,L blood sample is based on FLD of 169, instead of the usual UVD, with Xex =515 nm and Xn = 553 nm455. The lipoprotein in human serum can be separated into three fractions, VLDL, LDL and HDL, by a series of precipitation and centrifugation operations, carried out in the presence of EDTA and GSH (28) to avoid oxidation. In TBARS measurements carried out for serum by the fluorescence method (Xex =515 nm, Xa = 548 nm) it was found that 43% was bound to the lipoproteins, distributed as follows VLDL + LDL 27% and HDL 16%456. 

Table 8 Lipoprotein Distribution of Cyclosporine After Spiking Cyclosporine in Serum Samples from Either Fasted or Nonfasted Human Subjects...

A6. Alaupovic, P., Lee, D. M., and McConathy, W. J., Studies on the composition and structure of plasma lipoproteins. Distribution of lipoprotein families in major density classes of normal human plasma lipoproteins. Biochim. Biophys. Acta 260, 689-707 (1972). 

A22. Anderson, D. W., Nichols, A. V., Pan, S. S., and Lindgren, F. T., High density lipoprotein distribution. Resolution and determination of three major components in a normal population sample. Atherosclerosis 29, 161-179 (1978). 

P19. Polz, E., and Kostner, G. M., The binding of 32-glycoProte n-Ito human serum lipoproteins. Distribution among density fractions. FEBS Lett. 102, 183-186 (1979). 

Three examples of the clinical applications are presented alteration of lipoprotein distribution for a patient with acute hepatitis according to recovery of disease (Fig. 23), change of HDL subfractions for a patient with coronary heart disease by a drug therapy (Fig. 24), and diagnosis of the type for hyperlipidemia by HPLC patterns (Fig. 25). 

R. J. Dempsey, L. A. Cassis, D. G Davis, R. A. Lodder, Near-mfrared imaging and spectroscopy in stroke research Lipoprotein distribution and disease, available at http //www.pharm.uky.edu/ASRG/Wave/Lipo/lipo.htm... 

---