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Lipids adsorbents

Supernatant (residual proteins, lipids, adsorbed Preparation-1 SDC or SDS, some starch, etc.)... [Pg.53]

Penetration of membrane extracts at the n-decane-water interface occurred in a similar manner to penetration at the air-water interface. The limiting equilibrium spreading pressure was however lower, 33 mN m"l compared with 43 mN m Triton X-100 adsorbs more readily at the n-decane-water interface however, it would account for no more than an increase of 2mN m in surface pressure. Liposomal lipid adsorbs less readily than... [Pg.150]

Williams PF, Iwasaki Y, Ishihara K, Powell GL, Gilbert JA, Nakabayashi N, et al. Evaluation of the frictional properties of an elastomer with enhanced lipid-adsorbing ability. Proc Inst Mech Eng Part H J Eng Med I997 2I I(5) 359-68. [Pg.403]

Fig. 150. Thin-layer chromatogram of phospholipids and other polar lipids of bovine milk [159 a]. 1 carbohydrate (lactose) and protein, 2 sphingomyelin, 3 phosphatidyl choline, 4 phosphatidyl serine, 5 phosphatidyl inositol, 6 phosphatidyl ethanolamine, 7 cerebroside dihexoside ( ), 8 cerebroside mono-hexoside ( ), P fatty acids, 10 neutral lipids. Adsorbent Silica gel HR. Solvents I, chloroform-methanol-water-28% aqu. ammonia (130 + 70+8 + 0.5) II, chloroform-acetone-methanol-acetic acid-water (50 + 20 + 10 + 10 + 5). Time 40 min in each direction. Indicator charring with chromic sulphuric acid solution... Fig. 150. Thin-layer chromatogram of phospholipids and other polar lipids of bovine milk [159 a]. 1 carbohydrate (lactose) and protein, 2 sphingomyelin, 3 phosphatidyl choline, 4 phosphatidyl serine, 5 phosphatidyl inositol, 6 phosphatidyl ethanolamine, 7 cerebroside dihexoside ( ), 8 cerebroside mono-hexoside ( ), P fatty acids, 10 neutral lipids. Adsorbent Silica gel HR. Solvents I, chloroform-methanol-water-28% aqu. ammonia (130 + 70+8 + 0.5) II, chloroform-acetone-methanol-acetic acid-water (50 + 20 + 10 + 10 + 5). Time 40 min in each direction. Indicator charring with chromic sulphuric acid solution...
Table 6 shows that the surface of polycarbonate with adsorbed serum albumin is the most suitable one to be used in implant devices. The behavior of all lipids toward blood-polymer interaction is not similar and may change depending on the nature of lipid, net charge of the lipid-adsorbed surface and the lipid-protein/ lipid-platelet interaction at the interface. Under conditions of high cholesterol concentrations addition of vitamin C leads to suitable surface characteristics of polycarbonate. The question is how to garantee the preferential the albumin adsorption on an implant surface In works of Malmsten and Lassen [123] competitive adsorption at hydrophobic surfaces from binary protein solutions was... [Pg.805]

In contrast to surfactants, lipids adsorbed on hydrophilic surfaces can be expected to form planar bilayers, due to their large spontaneous radius of curvature. A double chain amphiphile forming a bilayer on silica was already discussed in chapter 3.1.2 in the context of 2H NMR investigations of water soluble amphiphiles. Bilayers from water insoluble lipid amphiphiles have been adsorbed to large spherical silica particles by condensation of unilamellar vesicles from aqueous solution, and a series of studies explored different NMR methods suitable for the measurement of lateral diffusion coefficients in such supported bilayers . [Pg.315]

Fig. VI-6. The force between two crossed cylinders coated with mica and carrying adsorbed bilayers of phosphatidylcholine lipids at 22°C. The solid symbols are for 1.2 mM salt while the open circles are for 10.9 roM salt. The solid curves are the DLVO theoretical calculations. The inset shows the effect of the van der Waals force at small separations the Hamaker constant is estimated from this to be 7 1 x 10 erg. In the absence of salt there is no double-layer force and the adhesive force is -1.0 mN/m. (From Ref. 66.)... Fig. VI-6. The force between two crossed cylinders coated with mica and carrying adsorbed bilayers of phosphatidylcholine lipids at 22°C. The solid symbols are for 1.2 mM salt while the open circles are for 10.9 roM salt. The solid curves are the DLVO theoretical calculations. The inset shows the effect of the van der Waals force at small separations the Hamaker constant is estimated from this to be 7 1 x 10 erg. In the absence of salt there is no double-layer force and the adhesive force is -1.0 mN/m. (From Ref. 66.)...
S-layer proteins adsorb preferentially at lipid films in the liquid-expanded phase [138] Crystalization is observed only at the liquid-condensed phase [138]... [Pg.365]

It has been shown by FM that the phase state of the lipid exerted a marked influence on S-layer protein crystallization [138]. When the l,2-dimyristoyl-OT-glycero-3-phospho-ethanolamine (DMPE) surface monolayer was in the phase-separated state between hquid-expanded and ordered, liquid-condensed phase, the S-layer protein of B. coagulans E38/vl was preferentially adsorbed at the boundary line between the two coexisting phases. The adsorption was dominated by hydrophobic and van der Waals interactions. The two-dimensional crystallization proceeded predominately underneath the liquid-condensed phase. Crystal growth was much slower under the liquid-expanded monolayer, and the entire interface was overgrown only after prolonged protein incubation. [Pg.367]

Step 2 - A patch pipet is removed from the solution, the polar head groups of the monolayer lipids are adsorbed to the interface while the fatty acid hydrophobic tails are exposed to the air ... [Pg.360]

The purified membrane bound Na,K-ATPase from kidney adsorbs to planar lipid bilayers and transient currents can be elicited upon release of caged ATP [99,100], The... [Pg.18]

TLC plates coated with the layer of polar adsorbent should be prewetted with a nonpolar solvent, such as benzene or n-heptane (n-hexane), to prevent deactivation of the adsorbent surface and to avoid glue up as a result of the penetration of the pores by lipid molecules and other impurities (i.e., wax). [Pg.253]

Preliminary purification of a starting band contaminated with plant oil should be performed by predevelopment with a nonpolar solvent such as benzene or n-heptane, delivered from the eluent container. Weakly retained ballast substances (e.g., lipids) move with the solvent to the edge of the adsorbent layer, covering the glass plate where the volatile solvent evaporates. The contaminants can then be removed (scraped out with the adsorbent) from the layer or adsorbed on the strip of blotting paper placed on the upper edge of the layer. [Pg.253]

The basic technology for the preparation of sample material is similar in all TLC preparations, irrespective of the origin of the hpid and specific preparation method for a variety of biological samples [43]. The most important factor is the solubihty of the sample. The lipid sample must be completely soluble in the dissolving solvent prior to the application and must be free from water. Either toluene or chloroform is commonly used as the solvent to dissolve hpid materials. The dissolving solvent should be nonpolar in namre and volatile at such a concentration that the hpid components in the sample are completely adsorbed throughout the entire thickness of the layer as quickly as possible. Although sample sizes as small as 1 to 10 pi can... [Pg.306]

A proteinaceous particulate material has been described that is effective as an oil spill-dispersant composition [1450]. The material is a grain product (such as oats) from which lipids are removed through organic solvent extraction. When such compositions are applied to an oil spill, they will adsorb oil, emulsify it, and finally, disperse it. Moreover, the compositions are substantially nontoxic. [Pg.307]

These assumptions were confirmed by the electrophoresis study of the washed creams. Electrophoresis of purified fat globules is a convenient method to characterize and quantify proteins adsorbed at the oil-water interface [35]. Electrophoretic data indicate that no casein, nor whey proteins, were adsorbed at the surface of raw-milk fat globule. Upon homogenization, caseins adsorbed preferentially at the lipid-water interface. In this case, bound a-lactalbumin accounted for 16% of the total interfacial proteins. Heat treatment also induced the interaction of proteins with the fat globules. The amount of bound proteins (per mg of lipids) for heated raw milk was half that for homogenized milk. [Pg.271]

An analogous apparatus to that of Ref. 9 was used to follow the effect of the lipid monolayer on the rate of electron transfer (ET). In this setup [47], an organic phase droplet (1,2-DCE) is continuously expanded into the aqueous phase, and the resulting current transient was monitored in the absence and presence of the adsorbed lipid mono-layer. The rate of ET was decreased as a function of the lipid concentration. [Pg.544]

The first membrane model to be widely accepted was that proposed by Danielli and Davson in 1935 [528]. On the basis of the observation that proteins could be adsorbed to oil droplets obtained from mackerel eggs and other research, the two scientists at University College in London proposed the sandwich of lipids model (Fig. 7.2), where a bilayer is covered on both sides by a layer of protein. The model underwent revisions over the years, as more was learned from electron microscopic and X-ray diffraction studies. It was eventually replaced in the 1970s by the current model of the membrane, known as the fluid mosaic model, proposed by Singer and Nicolson [529,530]. In the new model (Fig. 7.3), the lipid bilayer was retained, but the proteins were proposed to be globular and to freely float within the lipid bilayer, some spanning the entire bilayer. [Pg.121]


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See also in sourсe #XX -- [ Pg.306 ]




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