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Linker region functions

Structural properties of both AFA-PLN and WT-PLN bound to SER-CAla after reconstitution in a functional lipid bilayer environment were examined by 13C solid-state NMR.241 Chemical-shift assignments in all domains of AFA-PLN provide direct evidence for the presence of two terminal ot-helices connected by a linker region of reduced structural order that differs from previous findings on free PLN. A combination of the spectroscopic data with biophysical and biochemical data using flexible protein-protein docking simulations provides a structural basis for understanding the interaction between PLN and SERCala.244 Using a... [Pg.76]

R Dieckmann, M Pavela-Vrancic, E Pfeifer, El von Dohren, El Kleinkauf. The ade-nylation domain of tyrocidine synthetase 1 structural and functional role of the interdomain linker region and the (S/T)GT(T/S)GXPKG core sequence. Eur J Biochem 247 1074-1082, 1997. [Pg.35]

For rationally constructing domain-swapped proteins, one consideration is whether to include a linker region between the two domains. In general, hybrids with two autonomously functioning domains require significant distance between the two domains so that they can fold and... [Pg.59]

Fig. 3. uPA and plasmin cleavage sites in the linker region and in domain I of uPAR. The uPA cleavage sites in uPAR have been experimentally determined [17]. The plasmin (pli) cleavage sites have been deduced from its substrate specificity hydrolyzing peptide bonds with R or K residues on their carboxyl side. The amino acids of the functional epitope of mAb R3 are indicated [8],... [Pg.73]

Engineering members of the serine protease family which structurally consist of two homologous, stacked /1-barrels, Hopfner and coworkers designed a hybrid protease by fusing the N-terminal /1-barrel from trypsin to the C-terminal /1-barrel of factor Xa [74], The location of the fusion point in the linker region between the two /1-barrels was chosen following close inspection of the X-ray structures of trypsin and factor Xa. The resulting hybrid was shown to be fully functional as it hydrolyzed a broader but distinct spectrum of peptide substrates in comparison to the parental enzymes. [Pg.189]

In addition to Pgp-mediated MDR, there is also a non-Pgp-mediated MDR phenomenon. This comprises another ABC transporter subfamily that is called the MRP-family. At least seven members have been identified, and five (MRP 1, -3, -4, -5, and -6) of them are expressed at the BBB [(44,91) reviewed by Borst (92,93)]. The MRPs are membrane-fixed systems that vary in size from 1325 to 1545 amino acids (92). They comprise two transmembrane domains of six alpha hehces, a cytoplasmic linker region, and two intracellular ABCs. The linker region is essential for its transport function (94,95). In addition, MRP1, -2, -3, and -6 have an extra domain structure comprising five additional transmembrane-segments at the animo-end (92,96). Today, MRPs are considered amphipatic anion efflux pumps. [Pg.641]


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See also in sourсe #XX -- [ Pg.667 ]




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Linker regions

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