Linkage analysis periodate oxidation

Structural analysis of isolated amylose and amylopectin components has been carried out by standard methods based on methylation, periodate oxidation, and partial acid hydrolysis studies. Methylation and periodate oxidation studies established the linkage types and frequency of... 

The hydroxy- and oxo-compounds derived from di- and tri-saccharides after the sequence of periodate oxidation, sodium borohydride reduction, and acid hydrolysis have been identified by g.l.c. of their 0-trimethylsilyl ether derivatives, thus giving useful information for linkage analysis. ... 

There are two general methods for the determination of the character of the linkage of polysaccharides periodic acid oxidation (1,2) and methylation analysis (3). The present paper gives details of the possibilities for the utilization of the periodate oxidation. 

Studies of the chemical composition and biology of the lipopolysaccharide from Anacystis nidulans have been reported. The glycan component of this lipopolysaccharide is composed mainly of residues of o-mannose and small proportions of 3- and 4-0-methyl-D-mannose, D-galactose, D-glucose, L-fucose, 2-amino-2-deoxy-D-glucose, 2-amino-2-deoxy-D-mannose, and 3-deoxy-2-octulo-sonic acid. Methylation analysis and oxidation of the D-mannan chain with periodate revealed the presence of (1 3)- and (1 4)-linkages in the ratio 3 1. 

The structures of disaccharides and disaccharide alditols were revealed by periodate oxidation, reduction and analysis of the products by e.i.- and FAB-m.s. as their peracetyl or permethyl derivatives. The compounds gave simple m.s. with intense and characteristic ions from which the glycosidic linkage positions and ring size could be determined. ... 

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