Lilium longiflorum

Reynolds JD, DashekWV. Cytochemical analysis ofcallose distribution in Lilium longiflorum pollen tubes. Ann Bot 1976 40 409-416. 

A protocol for the light microscope radioautography of Lilium longiflorum pollen tubes labeled with [14C]-proline follows. This protocol, which does not require tissue embedding in paraffin or Paraplast, can be modified for paraffin-embedded tissues see Chapter 2). Thus, by employment of the protocol, together with the preceding introductory information in this chapter, one should be able to derive a protocol applicable to the cells or tissue in question. The performance of the protocol requires approval of an institution s Radiation Safety Officer. An inventory of incoming radionuclides, their presence in secondary containers, and their waste must be carefully recorded. The waste must be further broken down into solid waste, liquid waste, and animal carcasses to aid in its proper disposal. 

Germinate 20 mg fresh weight lots of Lilium longiflorum, cv. Ace pollen" in Petri dishes containing 10 mL medium supplemented with 0.1 p i[14C]-proline (pro) for 4 h at 27 2°C. 

Krolak JM, Taylor N, Dashek WV, Mills R. Azetidine-2-carboxylic acid-induced suppression of 14C-proline incorporation into cytoplasmic macromolecules and cell wall of Lilium longiflorum pollen, in Advances in Plant Reproductive Physiology (Malik CP, ed.), Kalyani Publishers, New Delhi, India, 1978, pp. 62-71. 

Incorporation of [3H]-Myo-Inositol into Germinating Lilium longiflorum Pollen... 

Source of Lilium longiflorum, cv. Ace pollen— greenhouse, botanical gardens, or supermarket at Easter... 

Procure, store, grow, and harvest Lilium longiflorum pollen, as in Dashek and Rosen (2). 

Ueda K, Kinoshita Y, Xu ZJ, Ide N, Ono M, Akahori Y, Tanaka I, Inoue M (2000) Unusual core histones specifically expressed in male gametic cells of Lilium longiflorum. Chromosoma 108 491—500 Unal E, Arbel-Eden A, Sattler U, Shroff R, Lichten M, Haber JE, Koshland D (2004) DNA damage response pathway uses histone modification to assemble a double-strand break-specific cohesin domain. Mol Cell 16 991-1002... 

Chen, M., and Loewus, F.A., 1977, myo-Inositol metabolism in Lilium longiflorum pollen. Uptake and incorporation of myo-inositol-2-3H. Plant Physiol. 59 653-657. 

Davies, M.D., and Dickinson, D.B., 1972, Properties of uridine diphosphoglucose dehydrogenase from pollen of Lilium longiflorum. Arch. Biochem. Biophys. 152 53-61. 

Dickinson, D.B., 1978, Influence of borate and pentaerythritol concentrations on germination and tube growth of Lilium longiflorum pollen. J. Am. Soc. Hortic. Sci. 103 413-416. 

Kroh, M., Labarca, C., and Loewus, F., 1971, Utilization of pistil exudates for pollen tube wall biosynthesis in Lilium longiflorum. In Heslop-Harrison, J. (ed.), Pollen Development and Physiology. Butterworths, London, pp. 273-278. 

Kroh, M., Miki-Hirosige, H., Rosen, W., and Loewus, F., 1970a, Inositol metabolism in plants. VII. Distribution and utilization of label from myo-inositol-U-14C and -2-3H by detached flowers and pistils of Lilium longiflorum. Plant Physiol. 45 86-91. 

Labarca, C., and Loewus, F., 1970, The nutritional role of pistil exudate in pollen tube wall formation in Lilium longiflorum. II. Production and utilization of exudate from stigma and stylar canal. Plant Physiol. 52 87-92. 

Sherman, W.R., Loewus, M.W., Pina, M.Z., and Wong, Y.-H.H., 1981, Studies on myo-inositol-1-phosphate synthase from Lilium longiflorum pollen, Neurospora crassa and bovine testis. Further evidence that a classical aldolase is not utilized. Biochim. Biophys. Acta 660 299-305. 

Loewus, M.W., Bedgar, D.L., and Loewus, F.A., 1984, 1 L-myo-inositol 1-phosphate synthase from pollen of Lilium longiflorum. An ordered sequential mechanism. J. Biol. Chem. 259 7644-7647. 

Helsper, J.P.F.G., Heemskerk, J.W.M. and Veerkamp, J.H., 1987, Cytosolic and particulate phosphatidylinositol phospholipase C activity in pollen of Lilium longiflorum. Physiol. Plant. 71 120-126. 

Lilium longiflorum (anthers, pollen) Liliaceae Brassinolide (1), Castasterone (3), Typhasterol (7), Teasterone-myristate (9) 3-Dehydroteasterone (31) 9, 44, 57... 

Gussin and McCormack presented evidence for the presence of trehalose 6-phosphate synthetase in the pollen of Lilium longiflorum. 

---