Lignin sulfonates with electrolyte solution

Elution of Lignin Sulfonates with Electrolyte Solution and Calibration of Columns... 

The effects caused by the electrolyte nature of lignin sulfonates are eliminated by using a 0.5M sodium chloride solution as eluent. This eluent is made 0.1M with respect to Tris-HCl and buffered to pH 8 with hydrochloric acid in order to dissolve the proteins used as calibration standards (Fig. 5). 

Figure 6 shows that the proteins used for calibration elute in the same way as lignin sulfonates, which justifies the use of proteins as calibration standards. A comparison between Figures 4 and 6 shows that elution with an electrolyte solution fractionates lignin sulfonates in the range 3 000-80 000 dalton, but that elution with water fractionates those in the range 20 000-100 000 dalton. 

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See also in sourсe #XX -- [ , , , ]

See also in sourсe #XX -- [ , , ]

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