Liquid light scattering detectors

Evaporative light scattering detector, liquid chromatography, 4 623 Evaporators... 

Lafosse, M., Dreux, M., Morin-Allory, L., and Colin, J. M., Some applications of a commercial light-scattering detector for liquid chromatography, J. HRC CC, 8, 39, 1985. 

Mourey, T. H. and Oppenheimer, L. H., Principles of operation of an evaporative light-scattering detector for liquid chromatography, Anal. Chem., 56, 2427, 1984. 

Mengerink, Y., De Man, H.C.J., Van der Wal, S. (1991). Use of an evaporative light scattering detector in reversed-phase high-performance liquid chromatography of oligomeric surfactants. J. Chromatogr. A 552(1-2), 593-604. 

Trathnigg, B., Kollroser, M.J. (1997). Liquid chromatography of polyethers using universal detectors V. Quantitative aspects in the analysis of low-molecular mass poly(ethylene glycols) and their derivatives by reversed-phase HPLC with an evaporative light scattering detector. J. Chromatogr. A 768, 223-238. 

Gaudin, K., Baillet, A., and Chaminade, P., Adaptation of an evaporative light-scattering detector to micro and capillary liquid chromatography and response assessment. Journal of Chromatography A 1051(1-2), 43-51, 2004. 

Bravi E, Perretti G, Montanan L. Fatty acids by high-perfomance liquid chromatography and evaporative light-scattering detector. Journal of Chromatography A 1134, 210-214, 2006. 

Fig. 42 Reversed-phase HPLC profiles of natural (top) and rearranged (bottom) butterfat triacylglycerols as obtained with the light-scattering detector. HPLC conditions Hewlett-Packard Model 1050 liquid chromatograph equipped with a Supelcosil LC-18 column (25 cm X 0.46-cm ID) coupled to a Varex ELSD II light-scattering detector. Solvent linear gradient of 10-90% propanol in acetonitrile at 25°C over a period of 90 min (1 ml/min) recording stopped at 70 min. Peak identification by carbon and double-bond numbers of triacylglycerols.

Ruiz-Sala et al. (129) described a reversed-phase HPLC method with a light-scattering detector for the analysis of TGs in milk fat. The identification of TGs was carried out by a combination of HPLC and gas-liquid chromatography (GLC), and was based on the equivalent carbon numbers and retention times of different standard TGs. Finally, quantitation of peak areas from HPLC chromatograms was carried out after applying a deconvolution program to the parts of chromatograms with poor resolution. 

FD Conforti, CH Harris, JT Rinehart. High performance liquid chromatographic analysis of wheat flour lipids using an evaporative light scattering detector. J Chromatogr 645 83-88, 1993. 

MN Vaghela, A Kilara. Quantitative analysis of phospholipids from whey protein concentrates by high performance liquid chromatography with a narrow-bore column and an evaporative light scattering detector. J Am Oil Chem Soc 72 729-733, 1995. 

McNabb TJ, Cremesti AE, Brown PR, Fischl AA. High-performance liquid chromatography/evaporative light-scattering detector techniques for neutral, polar, and acidic lipid classes a review of methods and detector models. Sem Food Anal 1999 4 53-70. 

The evaporative light-scattering detector (ELSD) was originally developed for use with high-performance liquid chromatography (HPLC) to detect nonvolatile compounds by mass rather than ultraviolet (UV) absorbance detection [1], The response is dependent on the light scattered from particles of the solute remaining after the mobile phase has evaporated and is proportional to the total amount of the solute. Because no chromophore is necessary, a response can be measured for any solute less volatile than the mobile phase. 

Wang, T. and Hammond, E.G. (1999) Fractionation of soybean phospholipids by high-performance liquid chromatography with an evaporative light scattering detector. J. Am. Oil Chem. Soc., 76, 1313-1321. 

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