Light scattering detector, high-performance liquid chromatography

Mengerink, Y., De Man, H.C.J., Van der Wal, S. (1991). Use of an evaporative light scattering detector in reversed-phase high-performance liquid chromatography of oligomeric surfactants. J. Chromatogr. A 552(1-2), 593-604. 

MN Vaghela, A Kilara. Quantitative analysis of phospholipids from whey protein concentrates by high performance liquid chromatography with a narrow-bore column and an evaporative light scattering detector. J Am Oil Chem Soc 72 729-733, 1995. 

McNabb TJ, Cremesti AE, Brown PR, Fischl AA. High-performance liquid chromatography/evaporative light-scattering detector techniques for neutral, polar, and acidic lipid classes a review of methods and detector models. Sem Food Anal 1999 4 53-70. 

This method uses the high-performance liquid chromatography (HPLC) equipment for sample handhng and requires molar mass sensitive detectors (such as light scattering and/or viscometry) to obtain a mean property values from each detector (Mw and/or IV, respectively). The FIA result from a concentration detector yields polymer content in a sample, which can also be determined with other well-established methods. The FIA approach requires expensive and well-maintained equipment, and will not save much time or solvent furthermore, no distribution information is available. 

The evaporative light-scattering detector (ELSD) was originally developed for use with high-performance liquid chromatography (HPLC) to detect nonvolatile compounds by mass rather than ultraviolet (UV) absorbance detection [1], The response is dependent on the light scattered from particles of the solute remaining after the mobile phase has evaporated and is proportional to the total amount of the solute. Because no chromophore is necessary, a response can be measured for any solute less volatile than the mobile phase. 

Wang, T. and Hammond, E.G. (1999) Fractionation of soybean phospholipids by high-performance liquid chromatography with an evaporative light scattering detector. J. Am. Oil Chem. Soc., 76, 1313-1321. 

Christie, W. W. (1992b) Detectors for high-performance liquid chromatography of lipids with special reference to evaporative light-scattering detection, in Advances in Lipid Methodology -One (ed. W. W. Christie), The Oily Press, Ayr, pp. 239-71. 

Lafosse, M., Dreux, M. and Morin-Allory, L. (1987) Application fields of a new evaporative light scattering detector for high-performance liquid chromatography and supercritical fluid chromatography. J. Chromatogr., 404, 95-105. 

Sas, B. Peys, E. Helsen, M. Efficient method for (lyso)phospholipid class separation by high-performance liquid chromatography using an evaporative light-scattering detector, J.ChromatogrJ, 1999, 864, 179-182. 

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