Light scattering detector, high-performance

Mengerink, Y., De Man, H.C.J., Van der Wal, S. (1991). Use of an evaporative light scattering detector in reversed-phase high-performance liquid chromatography of oligomeric surfactants. J. Chromatogr. A 552(1-2), 593-604. 

FD Conforti, CH Harris, JT Rinehart. High performance liquid chromatographic analysis of wheat flour lipids using an evaporative light scattering detector. J Chromatogr 645 83-88, 1993. 

MN Vaghela, A Kilara. Quantitative analysis of phospholipids from whey protein concentrates by high performance liquid chromatography with a narrow-bore column and an evaporative light scattering detector. J Am Oil Chem Soc 72 729-733, 1995. 

McNabb TJ, Cremesti AE, Brown PR, Fischl AA. High-performance liquid chromatography/evaporative light-scattering detector techniques for neutral, polar, and acidic lipid classes a review of methods and detector models. Sem Food Anal 1999 4 53-70. 

The evaporative light-scattering detector (ELSD) was originally developed for use with high-performance liquid chromatography (HPLC) to detect nonvolatile compounds by mass rather than ultraviolet (UV) absorbance detection [1], The response is dependent on the light scattered from particles of the solute remaining after the mobile phase has evaporated and is proportional to the total amount of the solute. Because no chromophore is necessary, a response can be measured for any solute less volatile than the mobile phase. 

Wang, T. and Hammond, E.G. (1999) Fractionation of soybean phospholipids by high-performance liquid chromatography with an evaporative light scattering detector. J. Am. Oil Chem. Soc., 76, 1313-1321. 

Fig. 3. Silver-ion high-performance liquid chromatogram of phenacyl esters of cyclic fatty acids derived from heated linseed oil. A column of Nucleosil 5SA (250 x 4.6 mm i.d. 5 pm particle size) was used in the silver ion form. The mobile phase was composed of dichloromethane-dichloroethane (50 50 vol/vol Solvent A) and dichloromethane-dichloroethane-acetonitrile (49 49 2, by vol Solvent B) the flow rate was 1 mL/min. There was a linear gradient from 100% A to 75% A/25% B over 50 min, then to 100% B over a further 5 min. An evaporative light-scattering detector was employed. Source Ref. 10.

Lafosse, M., Dreux, M. and Morin-Allory, L. (1987) Application fields of a new evaporative light scattering detector for high-performance liquid chromatography and supercritical fluid chromatography. J. Chromatogr., 404, 95-105. 

Makinen, M., Piironen, V. and Hopia, A. Postcolumn chemiluminescence, ultraviolet and evaporative light-scattering detectors in high-performance liquid chromatographic determination of triacylglycerol oxidation products. J. Chromatog. A. 734, 221-229 (1996)... 

Sas, B. Peys, E. Helsen, M. Efficient method for (lyso)phospholipid class separation by high-performance liquid chromatography using an evaporative light-scattering detector, J.ChromatogrJ, 1999, 864, 179-182. 

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