Light-responsive elements

Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B. 1998. Identification of UV/blue light response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Mol Biol 36 741-754. 

QUESTION 1 8.20 The mechanism by which light influences plant gene expression is referred to as photomorphogenesis. Because of serious technical problems with plant cell culture, relatively little is known about plant gene expression. However, certain DNA sequences, referred to as light-responsive elements (LRE), have been identified. On the basis of the gene expression patterns observed in animals, can... 

We have examined two complete chloroplast genome sequences (Nicotiana tabacum (12) and Marchantia polymorpha (13)) and some individual chloroplast genes searching for LRE-like matrices homologous to the light responsive elements found in plant nuclear light-inducible... 

The response to pathogen infection involves not only some of the cA-elements characterized for the UV-light response but also additional regulatory elements related to pathogen-associated stimuli. These include the H-box and a TGAC (W-box) sequence that interacts specifically with a WRKY H-boxes have been associated with stress induction... 

Weisshaar, B. et al.. Light-inducible and constitutively expressed DNA-binding proteins recognising a plant promoter element with functional relevance in light responsiveness. EMBO J., 10, Mil, 1991. 

Hartmann U, Sagasser M, Mehrtens F, Stracke R, Weisshaar B. 2005. Differential combinatorial interactions of cis-acting elements recognized by R2R3-MYB, BZIP, and BHLH factors control light-responsive and tissue specific activation of phenyl-propanoid biosynthesis genes. Plant Mol Biol 57 155-171. 

Lois, R., Dietrich, A., Hahlbrock, K. Schulz, W. (1989). A phenylalanine ammonia-lyase gene from parsley structure, regulation and identification of elicitor and light-responsive ds-acting elements. The EMBO Journal 8, 1641-8. 

Block, A., Dangl, J.L., Hahlbrock, K. Schulze, L.P. (1990). Functional borders, genetic fine structure, and distance rquirements of cis elements mediating light responsiveness of the parsley chalcone synthase promoter. Proceedings of the National Academy of Sciences (USA) 87, 5387-91. 

Fig. 5.1 Principle behind the CALUX bioassay for Ah-receptor agonists. Following binding of the agonist to the Ah-receptor, the complex will be transported to the nucleus and bind to a so-called dioxin responsive element, resulting in the increased transcription of the luciferase gene and production of luciferase. Following incubation this enzyme can subsequently be measured in cell lysates by a light producing reaction.

From the measured I-V characteristics of the test element group, presented in GB-A-2246662 above, only the presence or absence of the pn-junction in the light responsive region is detected Even if a photo-detector has insufficient spatial resolution caused by the excessive diffusion length of the minority charge carrier in the semiconductor layer in which the pn-junctions are formed, the insufficient space resolution is not detected from the I-V characteristics of the test element group, and this photo-detector is selected as a non-defective. 

In GB-A-2274739 (Mitsubishi Denki Kabushiki Kaisha, Japan, 03.08.94) a photo-detector is presented which includes a test element group that detects the spatial resolution of the light responsive region. 

The following are prominent lines in the visible region of the emission spectra of the elements listed. The lines can be used to identify the elements. What color is the light responsible for each line (a) lithium, 4603 A ... 

---