Light microscopy distributional

Muscle biopsy shows vacuolar myopathy of very severe degree affecting all fibers in Pompe s disease but of varying degree and distribution in childhood and adult AMD. In adult AMD, biopsy specimens from unaffected muscles may appear normal by light microscopy. The vacuoles contain PAS-positive material, a marker for glycogen. Electron microscopy shows abundant glycogen, both within membranous sacs, presumably lysosomes, and free in the cytoplasm. 

In B.C. they are usually separated distributionally, but intermediate forms occur in intermediate localities. In Japan they may be temporally separated.The allocation of Gymnodinium breve to Ptychodiscus depends on the presence of a pellicle. Although not seen with TEM it can be seen with light microscopy. Geographic distribution is closely linked to taxonomy for, although some toxin producers appear to be endemic in a restricted sense, closely similar forms occur elsewhere (e.g.p, brevis) or the same species may be known by different names in different regions ( Gyrodinium aureolum ). 

The structure (e.g., number, size, distribution) of fat crystals is difficult to analyze by common microscopy techniques (i.e., electron, polarized light), due to their dense and interconnected microstructure. Images of the internal structures of lipid-based foods can only be obtained by special manipulation of the sample. However, formation of thin sections (polarized light microscopy) or fractured planes (electron microscopy) still typically does not provide adequate resolution of the crystalline phase. Confocal laserscanning microscopy (CLSM), which is based on the detection of fluorescence produced by a dye system when a sample is illuminated with a krypton/argon mixed-gas laser, overcomes these problems. Bulk specimens can be used with CLSM to obtain high-resolution images of lipid crystalline structure in intricate detail. 

Because of this importance, different techniques have been developed to characterize the droplet size distribution in emulsions, each with its own pros and cons. Light microscopy, for example, is qualitative and only suited for particles larger than about 1 )im. When using electron microscopy, correct sample preparation is crucial to the examination and interpretation of the dispersions. The Coulter method is an indirect method which detects a... 

Petrographic data consist of light photomicrographs, electron micrographs, x-ray microanalyses, and observations on size and distribution of pyrite crystals. Light microscopy was carried out on all 57 sections, while electron microscopy and x-ray microanalysis were carried out on two sections, one from the Avery site (depth 210 cm) and one from the Barataria site (depth 106 cm). Micrographs and x-ray microanalysis of iron sulfides are shown in Figures 9 and 10, respectively. 

Catalyst preparation and inspection by microscopy. Preparation by impregnation with ammonium iron citrate and iron nitrate resulted in a homogeneous iron distribution as determined by light microscopy. Ammonium iron EDTA as a precursor yielded an eggshell distribution of the iron compound. Finely divided material deposited on the support was observed with Transmission Electron Microscopy in all catalysts. Tn addition to this, some material deposited next to the support was observed in catalysts ex nitrate. It was therefore decided to focus on the catalysts prepared with ammonium Fe(ITI) citrate. 

Microspheres intended for nasal administration need to be well characterized in terms of particle size distribution, since intranasal deposition of powder delivery systems is mostly determined by their aerodynamic properties and particle sizes. Commonly used methods for particle size determinations described in the literature are sieving methods [108], light microscopy [58], photon correlation spectroscopy [66], and laser diffractometry [25,41,53,93], The morphology of the microparticles (shape and surface) has been evaluated by optical, scanning, and transmission electron microscopy [66, 95],... 

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