LiChrospher

Figure 16.4 displays the separation of the resins of B. carterii and B. serrata, their hydrodistillates, and three commercially available olibanum essential oils on a Merck LiChrospher plate in the mobile phase heptane-diethylether-formic acid (7 + 3 + 0.3 v/v/v) without chamber saturation after derivatization with anisaldehyde reagent. 

Column L-Column LiChrospher-C18 HRC-ODS Inertsil ODS L-Column Zorbax SB-C8... 

HPLC column, Merck, LiChrospher 60 RP Select B, 5 itm, 150 x 4-mm i.d. Autosampler vials, 1.8-mL Assorted laboratory glassware Autopipettes, calibrated... 

Figure 4.24 Calibration curves for LiChrospher colunn. Calibration standards poly(styrene) and mobile phase tetrahydrofuran.

Figure 4.23 Molecular weight distribution of a carboxynethyl-cellulose sasple. Coluiui combination of LiChrospher Si-100 and Si-500 mobile phase 0.5 H aqueous sodium acetate, pH 6, and flow rate 0.5 ml/min.

FIGURE 9.3 Typical SCX-RAM column separation profile peaks (a) represent physical exclusion by pore size. Trapped retained biomolecules are separated by a gradient in the second step (b). Conditions column—LiChrospher 60 XDS (S03/Diol), 25 x 4 mm I.D., flow rate— 0.5 mL/min, gradient from 0 to 1M NaCl in 20 mM KH2P04 pH 2.5, containing 5% ACN in 30 min. Sample 100 pL Human Hemofiltrate (3.7mg/mL), UV detection at 214 nm. 

Fig. 2.3.6. Overlay of reversed-phase chromatograms of four commercial mixtures of alkylphenols and alkyl phenolethoxylates f-OP, OP[EO]8/9) 4-NP, and NP[EOJio. Column 125 X 4 mm2 Lichrospher RP-18 (5 pm), isocratic elution 1.0 mL min-1, mobile-phase 8 2... 

Figure 4.3 Effect of particle diameter on plate height. (Reproduced from Lichrospher Lichroprep Sorbents Tailored for Cost Effective Chromatography, EM Separations, Gibbstown. With permission from Merck kGaA, Darmstadt, Germany, and EMD Chemicals, Inc.)...

Lichrospher Lichroprep Sorbents Tailored for Cost Effective Chromatography, EM Separations, Gibbstown. 

HPLC analyses were performed using a Jasco 880-PU pump equipped with a Jasco 870-UV detector and a LiChrospher 100 RP-18 (5 pm) in LiChroCART 125-4 column along... 

Mancinelli and colleagues (1999) reported the simultaneous determination of 3-4-methylenedioxymethamphetamine (MDMA), N-ethyl-3,4-methylenedioxyamphetamine (MDEA), methylenedioxyamphetamine (MDA), and N-methyl-l-(l,3-benzdioxol-5-yl)-2-butamine (MBDB) by RP-HPLC with fluorometric detection. The method required a 100 pi sample volume of serum, urine, or saliva separation was achieved on a LiChrospher lOORP-18 analytical column using an acetonitrile/water mobile phase (pH 11.4) set at a flow rate of 1 ml/min. The LLOQ and LLOD were 50 and 10 ng/ml respectively. 

For TRP-2 analysis, a reversed phase gradient method already developed (13) was slightly modified for our purpose. We used a Waters Alliance 2695 separations module equipped with a Waters 996 PDA detector and a CC125/3 Lichrospher 100-5 RP-18 column (Macherey und Nagel, Diiren, Germany) at 30°C and a flow rate of 0.5 mL/min. 

Fig. 2. Effect of silica pore structure on separation. Stationary phases are 10 LiChrospher SI 100, SI 00, SI 1000, and SI 4000 having 100, 500, 1000. and 4000 A mean pore diameter, respectively. Rowrate and inlet pressure are 5 ml/min and 125 bar, respectively. Sample components I, benzene 2. diphenyl 3, m-terphenyl 4, m-quaterphenyl 5, m ipiliiqiicphcnyl 6, m-sexiphenyl. (Cotiuesy of Merck AO.)...

Silica is the most frequently used adsorbent and for use in HPLC it is sold under a variety of brand names such as LiChrosorb. LiChrospher, Nucleosil, Partisil, Porasil, Spherisorb, Spherosil, and Zorbax. For adsorption chromatography, silicas with a relatively large specific surface area (S0-4S0 m /g), a large pore volume (0.7-1.2 ml/g), ahd a moderate mean pore diameter (50-250 A) are used. 

FIG U RE 1.13 Gradient separation of polypeptides on silica rod column and particle-packed columns. Mobile phase velocity 4mm/s, gradient 5%-60% ACN in the presence of TFA, gradient time 5min, columns (a) silica rod column, (b) Capcellpak SG (5 pm), (c) LiChrospher WP 300 RP-18e (5 pm), (d) nonporous NPS-ODS-1 HPLC column (1.5pm) (e) polymer-based TSKgel Octadecyl-NPR (2.5pm). (Reprinted from Minakuchi, H. et al., J. Chromatogr. A, 828, 83, 1998. Copyright 1998, with permission from Elsevier.)... 

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