LiChrospher Select

LiChrosorb Select B Spherisorb Ca LiChrospher Select B ymc J sphere mso... 

Even that would not be so bad, if the metal content of the original silica remains constant. However, this is not the case. Thus, the batch-to-batch reproducibility of stationary phases based on non-metal-free silica (the English-speaking literature often addresses Type A silica) is much worse than that of metal-free silica (so-called Type B silica). But here too, some separations require additional metal interactions and in certain cases it is quite possible that the separation will only work on Type A silica. So even today some users still swear on their LiChrospher, Select B or Spherisorb ODS 1. 

Our opinion Also think about, for example, Hypersil ODS, LiChrospher Select B 60, Superspher or Cosmosil NAP additionally, for example, Jupiter, Zorbax SB 300 as well as Nucleosil 50 - then orthogonality is given for all cases. 

The similarity of the Cg phases LiChrospher Select B, Superspher Select B, and Zorbax SB Cg is clearly evident... 

As is known, liChrospher and Superspher are essentially identical, the only difference being in their partide size (5 im compared to 4 im). The hexagons are nearly identical. In the case of LiChrospher Select B and Superspher Select B, they are again nearly the same. 

HPLC column, Merck, LiChrospher 60 RP Select B, 5 itm, 150 x 4-mm i.d. Autosampler vials, 1.8-mL Assorted laboratory glassware Autopipettes, calibrated... 

FLU, 7-OHFLU Sheep, muscle, liver, kidney, fat LLE 150 X 4 5-/xm LiChrospher RP Select B... 

CF, TB, TP, preservatives, and antioxidants HPLC-UV. Column LiChrospher 60 RP-Select B. 

Figure 6.1 UV chromatograms of the test mixture of four / -hydroxybenzoic acid esters (1, methyl 2, ethyl 3, propyl 4, butyl) after the column and after the NMR flow cell at flow rates of (a) 1.0 and (b) 0.1 ml/min conditions column, LiChrospher RP select B, 125 x 4 mm id, 5 Jim, spectrometer, Bruker DRX 600 probe head, 4 mm z-gradient LC probe, active volume 120 a1 eluents, acetonitrile (A) and D2O (B) gradient, t = Omin A/B (40/60), t = 8 min A/B (70/30) at a flow rate of 1.0 ml/min and t = 80 min A/B (70/30) at a flow rate of 0.1 ml/min...

Guard column 25x4 mm cartridge packed with LiChrospher 60 RP select B, 5 pm (Merck)... 

To check the selectivity with different chromatographic systems the separation with LiChrospher RP-18 is compared with the gradient separation with two different adsorbents. For LiChrospher RP-8 the separation with methanol-water is shown in... 

Lor analyses, two sets of HPLC conditions were used, depending on whether UV (285 nm) or MS (electrospray ionization on a triple quadrupole system) was to be used for detection. Lor MS detection, analyses were conducted using a 125 x 4 mm (5 pm) Lichrospher 60 RP-Select B column. Eluent A was 800 ml water/200ml acetonitrile/3.08 g ammonium acetate adjusted to pH 6.5 with acetic acid. Eluent B was 500 ml water/500ml acetonitrile/1.93 g ammonium acetate adjusted to pH 6.5 with acetic acid. The gradient was run at 0.5ml/min as follows from 0-25.0 min, 100% A from 25.0-35.0 min, 100% A-100% B from 35.0-45.0 min, 100% B from... 

Figure 10.10 Stationary phase for basic compounds. Conditions sample, adenine derivatives columns, 25cm x 4mm i.d. stationary phase left, LiChroshper 100 RP-18, 5 m (with high separation performance and good peak symmetry for neutral compounds) stationary phase right, LiChrospher 60 RP-Select B, 5 im mobile phase, 1.5mlmin water-methanol (25 75) UV detector, 260 nm.

Figure 19.9 Analysis of explosives at a UV wavelength change of 2 nm. Conditions column, 25 cm x 4.0 mm i.d. stationary phase, LiChrospher 60 RP-Select B, 5gm mobile phase, Imimin water-acetonitrile (30 70). Peaks 1=octogen 2 = hexogen 3 tetryl 4 = trinitrotoluene 5 = nitropenta.

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