Separator LiChrospher Diol

P-adrenergic blocking agent and various enantiomers Vancomycin bonded on LiChrospher diol silica, 5 pm Methanol-acetonitrile-acetic acid-triethylamine (80-20 20-80 0.1-0.3 0.1-0.4) 355 mm x 75 pm i.d. 265 mm effective length, chiral separation... 

Figure 7 Separation of carbamate pesticides on a 4.6x250 mm, 5 xmoll Lichrospher Diol column with 2.5 ml min of 5%...

FIGURE 9.3 Typical SCX-RAM column separation profile peaks (a) represent physical exclusion by pore size. Trapped retained biomolecules are separated by a gradient in the second step (b). Conditions column—LiChrospher 60 XDS (S03/Diol), 25 x 4 mm I.D., flow rate— 0.5 mL/min, gradient from 0 to 1M NaCl in 20 mM KH2P04 pH 2.5, containing 5% ACN in 30 min. Sample 100 pL Human Hemofiltrate (3.7mg/mL), UV detection at 214 nm. 

Benzoin acetate, methylben-zoin, Troger s base, tra/w-stilbene oxide and 1,1 -binaphthyl-2,2 -diol Aminopropyl silica gel (LiChrospher 1000, 5 pm) coated with helically chiral poly(diphenyl-2-pyridylmethyl methacrylate) (PDPM) Methanolic solution of ammonium acetate (2.5 mM, pH 4.5) 300 mm x 100 pm i.d. 200 mm effective length, chiral separation 163... 

Silica-based SEC columns commercially available at present are summarized in Table 2, together with their basic properties. LiChrospher 100 Diol series have also been used for many years and cited in published papers [ref. 29-33]. The other three are newly introduced. In Table 2, column efficiency of the columns is not shown because this factor, which is usually obtained by use of ethylene glycol as a sample, does not always reflect the separation efficiency of the columns for biologically complex samples. 

Figure 3. The size-exclusion chromatographic separation of a standard protein mixture on a Hibar RT LiChrospher 500 DIOL column. COhimn size, 250 X 4 mm (2 columns) eluant, 50 mM H3PO4 adjusted to pH 7.0 with 2.0 M NaOH -r 1% SDS flow-rate, 0.2 ml/min pressure, 15 kg/cm chart speed, 2.5 mnVntin detection, UV 208 nraO.08 AUFS. Samples, (1) albumin (bovine serum = 68 (KK)) (2) chymo-trypsinogen A (25 000) (3) cytochrome c (12 500) (4) alanine (89).

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