Libraries tags, oligonucleotide

Figure 1.10. Encoded combinatorial libraries by (A) sequenceable chemical tags (oligonucleotides and peptides) or by (B) non-sequenceable chemical tags (haloaromatic phenol ethers).

To identify an individual enriched ligand from synthetic chemical libraries (see the chapter by Zhao and Lam), the number of variants used is limited by the amount of material which is needed for sequence analysis. The use of smaller beads has been made possible by inclusion of an encryption tag, e.g. an oligonucleotide, the sequence of which can be determined after PCR [44,45], The size of such banks is, all the same, similar to phage-display libraries with some 10s molecular species per ml, using 10 pm beads [46],... 

Molecular tags overcome the chemical sensitivity and orthogonal protection required by both peptide and oligonucleotide encoding. Two such methods have been developed and applied to the synthesis of both peptide and nonoligomeric libraries. One of these [34] approaches uses chemically robust haloaromatic tags which are used in a binary encoding... 

The first encoding approaches used peptides (177) or oligonucleotides (178) as tags. We will consider two reported examples of resin-bound constructs able to support peptide-encoded (7.37, Fig. 7.25) (177) and oligonucleotide-encoded (7.38, Fig. 7.25) (179) peptide or peptidomimetic library synthesis. They both contain a core moiety, derived from L-lysine (7.37) or L-serine (7.38), which simultaneously multiphes the... 

Peptide nucleic acids (PNAs) are oligonucleotide-like molecules that have their DNA backbone removed and replaced with an achiral polyamide backbone that can hybridize with DNA through strand displacement (58). PNA tags have been used in the development of tagged libraries that allow for the spatially addressable localization and identification of probes on a DNA microarray. 

Clark, M.D., S. Hennig, R. Herwig, S.W. Clifton, M.A. Marra, H. Lehrach, S.L. Johnson and the WU-GSC EST Group. An oligonucleotide fingerprint normalized and expressed sequence tag characterized zebrafish cDNA library. Genome Res. 11 1594-1602, 2001. 

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