Leaf impressions

Density of trichomes and stomata -- Densities of trichomes and stomata were determined from positive leaf impressions by the silicone rubber technique of Sampson (1961). A total of eight areas were sampled randomly on each of four leaf replicas. 

The production of C5-1 by co-infiltration illustrates the impressive capacity of this method. Results presented in Fig. 1.4 show that the production of C5-1 in detached alfalfa leaves was validated within 5 days from infiltration. In these experiments, different bacteria bearing the light- and the heavy-chain constructs were used to infect the cells. Most of the infected cells were occupied by both strains, and a protein corresponding to fully assembled C5-1 was detected in the infiltrated leaf extract This result demonstrates the potential of agroinfiltration for testing the adequate expression and assembly of complex proteins in alfalfa leaves using different Agrobacterium strains. 

My feelings of serenity continued on the next day and continue even today. I was very impressed by the unity in nature, down to the image of a tree reflected in the veins of a lettuce leaf. My general feeling of peace has continued to the... 

Using rose-leaf cutters or a small sharp knife, cut out pointed-edge petals, making three sizes small, medium, and large. Using a silicone veiner (see page 232), make a slight impression on each petal. 

Nath, S.C. and Baruah, A.K.S. (1994) Eugenol as the major component of the leaf oil of Cinnamomum impress-inervium Meissn. Journal of Essential Oil Research 6, 211-212. 

Owing to their characteristic minty-fruity notes, reminiscent of blackcurrants, buchu leaf oils are well appreciated in the composition of flavourings and fragrances. 8-Mercapto-p-menthan-3-one and its thiolacetate have been described by Sundt et al. [98] and simultaneously by Lamparsky et al. ]99]as impact flavouring compounds of the flavour of cassis. They have so far not been identified in blackcurrant. All four stereoisomers of 8-mercapto-p-menthan-3-one exhibit distinct and characteristic sensory impressions ]100]. 

Production By steam distillation of the aerial parts of the parsley plant, including the unripe seeds. Composition A major part of the oil consists of monoterpene hydrocarbons such as a- andP- pinenes, myrcene, limonene, P-phellandrene (see p-mentha-dienes). The occurrence of p-mentha-1,3,8-triene (usually between 20 and 30%) (C,oH,4, Mr 134.22) is characteristic and mainly responsible for Ae organoleptic impression. According to recent investigations a series of other trace components with widely varying structures also contributes to the typical parsley odor. The leaf oil also contains lower concentrations of those compounds that constitute the main components of parsley seed oil (see below). 

Superhydrophobic surfaces (water contact angles higher than 150°) can only be achieved by a combination of hydrophobicity (low surface energy materials) with appropriate surface texture. In nature one can find an array of impressive and elegant examples of superhydrophobic surfaces. For example, on a lotus leaf rain drops bounce off after impact, then entirely roil off the lotus leaf and drag along any dirt particles, without leaving residues. 

Analysis of pure gases by LEAF is much less developed. Nevertheless, some very impressive results have been demonstrated. Traces of Pb and Tl in the air of a normal laboratory and a class-100 clean room were detected by LEAF. Air from the room was pumped through a tantalum jet, fixed in a hole inside a graphite tube. The heavy particles impacted onto the tube wall opposite the jet. After pumping a certain volume of ambient air in a particular time interval the impacted metal traces were detected by graphite furnace LEAF. LODs of 0.1 pg/m and 0.01 pg/m for Pb and Tl. re.spectively, were achieved. The Pb LOD is two orders of magnitude better than the LOD for graphite furnace AAS. The measured concentrations of Pb and Tl in laboratory air were 1.25 ng/m and 4.3 pg/m , respectively. The same values in clean-room air were about 0.3-0.6 ng/m and 0.9-1.0pg/m. The measured concentrations of the same metals inside a clean... 

In the course of our study on saponin in leaf protein concentrates we had occasion to examine a number of common vegetable food products regarding their saponin contents. We were impressed by the very high saponin contents of commercially grown alfalfa sprouts found in the market place and accordingly have made further study on possible methods to minimize the saponin in alfalfa... 

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