Laser-induced thrombosis

Thrombus formation in rat or rabbit mesenteric arterioles or venules is induced by laser beams. The test can be performed in normal or pretreated (induction 

4 W Argon laser (Spectra Physics, Darmstadt, FRG) wave length 514 nm, energy below the objective 15 mW, duration of exposure 1/30 or 1/15 s 

Videoanalyzer and correlator to determine blood flow velocity. 

Male Sprague Dawley or spontaneously hypertensive stroke prone Wistar or Lewis rats with adjuvant induced arthritis weighing 150-300 g or New Zealand rabbits with arteriosclerosis induced by cholesterol feeding for 3 months are used. The animals receive the test compound by oral, intravenous, intraperitoneal, or subcutaneous administration. Control animals are treated with vehicle alone. Prior to thrombus induction, the animals are pretreated by s.c. injection of 

1 mg/kg atropine sulfate solution and anaesthetized by intraperitoneal administration of 100 mg/kg ketamine hydrochloride and 4 mg/kg xylazine. 

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Weichert W, Breddin HK (1987) Effect of naftidrofuryl and dipyridamole on laser-induced thrombosis in rat mesenteric vessels. Vasa 16 377-380... 

Antithrombotic activity in the laser-induced thrombosis test was observed in mice orally administered a juice extract of fresh rosemary at doses of 3.85 ml/kg. No effect on vasodilation was observed (Yamamoto et al. 2005). 

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