Lambda mutants

Katsura I, Structure and function of the major tail protein of bacteriophage lambda, Mutants having small major tail protein molecules in their virion, J. Mol. Biol., 146 493-512, 1981. 

Nelson, H. C. and Sauer, R. T. (1986). Interaction of mutant lambda repressors with operator and non-operator DNA. /. Mol. Biol. 192,27-38. 

The fastest-folding small proteins generally fold on much slower time scales than the time scale of formation of secondary structure. The speed record is currently held by lambda(6-85), a truncated, monomeric form of the N-terminal domain of lambda repressor, which refolds with a half-life of approximately 140 fjis. A thermostable lambda(6-85) variant with alanine substituted for glycine residues 46 and 48 in the third helix folds faster in dilute solutions of de-naturant, with an extrapolated half-life of less than 10 /us in water.13 Cold-shock protein CspB from Bacillus subtilis folds in about 1 ms.61 Engineered mutants of the P22 Arc repressor62 and CI263 fold in a fraction of a millisecond. 

McElroyAE, Bogler A, WeisbaumD, etal. Uptake, metabolism, mutant frequencies and mutational spectra in [lambda] transgenic medaka embryos exposed to benzo[[alpha]] pyrene dosed sediments. Mar Environ Res. 2006 62(Suppl. 1) S273-S277. 

Costantino, N. and Court, D.L. (2003) Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants. Proc. Natl. Acad. Sci. U.S.A., 100 (26), 15748-15753. 

The study of the mechanism of assembly of a phage particle was first approached with coliphage T4 making use of the electron microscope and conditional lethal mutants. > Since then, the most studied phages, among those that contain double-stranded DNA, have been T4, lambda and P22. 

Mertz P, Yu L, Sikkink R, Rusnak F. 1997. Kinetic and spectroscopic analyses of mutants of a conserved histidine in the metallophosphatases calcineurin and lambda protein phosphatase. J Biol Chem 272 21296-21302. 

Parkinson, J. S., Huskey, R. J. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J. molec. Biol. 56, 369-384 (1971). 

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