Lactate dehydrogenase temperature

Amplification of the sensitivity of substrate or co-en me recycling is especially efficient in thermometric analysis since all the reactions involved frequently contribute to increasing the overall temperature change. One case in point is the determination of lactate or pyruvate by substrate recycling using co-immobilized lactate oxidase and lactate dehydrogenase [160]. 

Pinhero, R. G., Copp, L. J., Amaya, C. -L., Marangoni, A. G., Yada, R. Y. (2007). Roles of Alcohol dehydrogenase. Lactate dehydrogenase and Pyruvate decarboxylase in Low Temperature Sweetening in a tolerant and susceptible varieties of Potato (Solanum tuberosum). Physiologia Plant, 130(2), 230-239. 

Miller et al. [3.83] freeze-dried lactate dehydrogenase (LDH) in the presence of trehalose and trehalose plus sodium tetraborate (TST) to stabilize LDH for storage at high humidity (100%) or warm temperature (45 °C). The freeze-dried LDH with TST had a considerably higher Tg than with trehalose alone and was more stable for several weeks under the conditions given above. 

Klyachko, O.S. and Ozemyuk, N.D. (1991). Temperature adaptations of metabolism the influence of temperature on kinetic parameters of lactate dehydrogenase (Km) in fish of different species during growth (In Russian). Doklady Academii Nauk SSSR 319,1252-1255. 

Tsukuda, H. (1975). Temperature dependency of the relative activities of liver lactate dehydrogenase isozymes in goldfish acclimated to different temperatures. Comparative Biochemistry and Physiology 52B, 343-345. 

Valkirs, A. (1978). Temperature and pH effects on catalytic properties of lactate dehydrogenase from pelagic fish. Comparative Biochemistry and Physiology 59A, 31-36. 

Lactate dehydrogenase a model enzyme for studying adaptation to temperature... 

Coppes, Z.L., and G.N. Somero (1990). Temperature-adaptive differences between the M4-lactate dehydrogenases of stenothermal and eurythermal Sciaenid fishes. J. Exp. Zool. 254 127-131. 

Yancey, P.H., and G.N. Somero (1978). Temperature dependence of intracellular pH its role in the conservation of pyruvate apparent Km values of vertebrate lactate dehydrogenases. J. Comp. Physiol. 125B 129-134. 

A number of other lines of evidence also suggested that there may be another mode-of-action for BTI poisoning by injection other than its known, general cytolytic activity (3,22-24). Using the appearance of cytosolic lactate dehydrogenase (LDH) in insect hemolymph post-injection as a marker for cytotoxicity (36-37), we found that dissolved BTI 6-endotoxin was a potent cytotoxin. When T. ni, however, were injected with dissolved BTI 5-endotoxin and then incubated at 28, 15, and 9°, there was an increase in the LD50 with a decrease in temperature (Figure 2) but the LDH levels at 3.5 PPM BTI were unaffected by temperature. 

Hatley, R. H. M. and Franks, F., Denaturation of lactate dehydrogenase at subzero temperatures. CryoLetters 7, 226-233 (1986). 

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