Lactate dehydrogenase isozymes

Assay of plasma enzymes aids diagnosis and prognosis. For example, a myocardial infarction elevates serum levels of lactate dehydrogenase isozyme I,. 

Derm = dermal Endocr = endocrine F = female (G) = gavage (GO) = gavage in oil Gastro = gastrointestinal Hemato = hematological hr = hour(s) LD50 = lethal dose, 50% kill LDH-C4 = lactate dehydrogenase isozyme C4 LOAEL = lowest-observed-adverse-effect level M = male ... 

Plasma hormones and enzymes of testicular origin were used as markers for evaluation of acute testicular toxicity in rats treated with 1,3-DNB. Lactate dehydrogenase isozyme C4 (LDH-C4) and ABP were both elevated after treatment with doses between 10 and 25 mg/kg of 1,3-DNB (Reader et al. 1991). Testosterone levels were reduced after treatment with 10 and 32 mg/kg of 1,3-DNB (Reader etal. 1991 Rehnberg et al. 1988). 

Tsukuda, H. (1975). Temperature dependency of the relative activities of liver lactate dehydrogenase isozymes in goldfish acclimated to different temperatures. Comparative Biochemistry and Physiology 52B, 343-345. 

Predict its most likely protein translation by submitting the DNA sequence along with the following amino acid sequences of human lactate dehydrogenase isozymes to Procustes analyses. 

Itoh, R., and H. K. Ozasa. 1985. Changes in serum lactate dehydrogenase isozyme X activity observed after cadmium administration. Toxicology Letters 28 151-154. 

Miller DR, Keeton JT, Acuff GR, Prochaska JF (2003). Verification of cooking endpoint temperatures in beef by immunoassay of lactate dehydrogenase isozyme 5. J. Food Saf., 68(6) 2076-2079. 

Evans SA Shore JD (1980) The role of zinc-bound water in liver alcohol dehydrogenase catalysis. J Biol Chem 255 1509-1514 Eventoff W, Rossmann MG, Taylor SS, Torff H-J, Meyer H, Keil W, Kiltz H-H (1977) Structural adaptations of lactate dehydrogenase isozymes. Proc Natl Acad Sci USA 74 2677-2681 Fisher HF, Conn EE, Vennesland B, Westheimer FH (1953) The enzymatic transfer of hydrogen I. The reaction catalyzed by alcohol... 

Fig. 82. Scheme (zymograms) of changes in lactate dehydrogenase isozymes during development of mouse organs. Numbers along abscissa indicate days before (-) and after (+) birth (Markert and Ursprung, 1962). 

Eventoff, W., Rossmann, M. G., Taylor, S. S., Torff, H-S., Meyer, H., Keil, W., and Kiltz, H-H., 1977, Structural adaptations of lactate dehydrogenase isozymes, Proc. Natl. 

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