Lactate dehydrogenase, chromatography

Ghose, S. and Mattiasson, B., Evaluation of displacement chromatography for the recovery of lactate dehydrogenase from beef heart under scale-up conditions,. Chromatogr., 547, 145, 1991. 

Displacement chromatography has also been carried out in dye affinity chromatographic systems for the purification of lactate dehydrogenase (LDH).59 In that study, polyethyleneimine (PEI) was employed as a displacer on dye affinity matrices prepared by immobilizing Cibacron Blue 3GA or Procion Red HE-3B. 

Lali, A., Kaul, R., Galaev, I. Yu., and Mattiasson, B. (1997). Purification of L-lactate dehydrogenase from crude homogenate of porcine muscle by expanded bed affinity chromatography (EBAC). Iso. Purif. 2, 289-300. 

Enzyme activity can be assayed using the same detection methods discussed above to determine metabolite concentrations. Spectroscopic methods are very important since they allow the progress of reaction to be monitored directly, which is much more convenient than sampling methods which may be based on chromatography, electrophoresis or the use of radioactive isotopes. As an example, the activity of lactate dehydrogenase can be assayed by following the reduction of NAD+ to NADH from the absorbance increase at 340 nm... 

Figure 14.8. Bioelution of LDH isoenzymes from a Sepharose AMP affinity column using a concave NADH gradient. [Reprinted, by permission, from P. Brodelius and K. Mosbach, FEBS Lett. 35, 1973, 223-226. Separation of the Isoenzymes of Lactate Dehydrogenase by Affinity Chromatography Using an Immobilized AMP-Analogue . The exclusive for all languages and countries is vested in the Federation of European Biochemical Societies.]...

D7. Denton, M. S., Bostick, W. D., Dinsmore, S. R., and Mrochek, J. E., Continuously referenced, on-line monitoring of creatine kinase and lactate dehydrogenase isoenzymes for use in clinical diagnostics. In "Biological/Biomedical Applications of Liquid Chromatography H (G. L. Hawk, ed.), pp. 165-191. Dekker, New York, 1979. 

Chaga G., Hopp J. and Nelson P. 1999. Immobilized metal ion affinity chromatography on Co -carboxymethylaspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle, Biotechnol. Appl. Biochem., 29(Pt 1), 19. 

Lactate dehydrogenase (from dogfish. Beef musele) [9001-60-9] Mr 140,000 [EC 1.1.1.27]. A forty-fold pirrification of the dehydrogenase is effected by affinity chromatography using Sepharose 4B coupled to 8-(6-aminohexyl)aniino-5 -AMP or -NAD. [Lees et al. Arch Biochem Biophys 163 561 1974, Pesce et al. J Biol Chem 239 1753 1964.]... 

WiELAND and Determann [191] have separated lactate-dehydrogenase isoenzyme and AMP, ADP and ATP from one another on DEAE-Sephadex layers, using gradient elution chromatography (p. 90). 

Affinity chromatographic purification 243 of lactic acid dehydrogenase Isolation of lactate dehydrogenase 191 isoenzyme X by affinity chromatography... 

Affinity chromatography of bacterial 244 lactate dehydrogenases separation of D-and L-specific enzymes by alteration of elution conditions Purification of cathepsin D by 245... 

Garg N, Galaev lY, Mattiasson B. Polymer-shielded dye affinity chromatography of lactate dehydrogenase from porcine muscle in an expanded bed system. Bioseparation 1996 6 193-9. 

Oxalic acid (8) Application of affinity chromatography to turnover studies of the lactate dehydrogenase isoenzymes Purification of lactate dehydrogenase 89 90... 

Galaev, I. Y. and Mattiasson, B. (1993). Effect of synthetic-polymers, poly(N-vinyl pyrrolidone) and poly(N-vinyl caprolactam), on elution of lactate-dehydrogenase bound to blue Sepharose. Journal of Chromatography, 648,367-371. 

Bylund, D., Samskog, J., Markides, K.E. and Jacobsson, S.P., Classification of lactate dehydrogenase of different origin by liquid chromatography-mass spectrometry and multivariate analysis. J. Am. Soc. Mass Spectrom., 14, 236-240 (2003). 

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