Kinases pyruvate kinase

Glycolysis Glyceraldehyde-3-phosphate dehydrogenase Phosphoglycerate kinase Pyruvate kinase... 

A tri-enzymatic sensing layer based on kinase-oxidase activities for the detection of acetate was also described. A reaction sequence using acetate kinase, pyruvate kinase and pyruvate oxidase enabled the production of H2O2 in response to acetate injection in the range 10 pM - 100 mM59. 

GMP NADH Disappearance GMP Kinase, Pyruvate Kinase, and Lactate Dehydrogenase Hypoxanthine-Guanine Phosphoribosyl-transferase ... 

ADP as a substrate in enzyme reactions, ADENYLATE KINASE (or MYOKINASE) ATP SYNTHASE CREATINE KINASE NUCLEOSIDE DIPHOSPHATE KINASE PHOSPHOGLYCERATE KINASE PYRUVATE KINASE RIBONUCLEOTIDE REDUCTASE SULFATE ADENYLYLTRANSFERASE (ADP) [ADP]/[ATP] ratio,... 

PYRUVATE CARBOXYLASE PYRUVATE DEHYDROGENASE KINASE PYRUVATE KINASE PYRUVATE, WATER DIKINASE RESTRICTION ENZYMES RHODOPSIN KINASE RIBOFLAVIN KINASE RIBONUCLEOTIDE REDUCTASE... 

Acetic acid Acetate kinase + pyruvate kinase + lactate dehydrogenase... 

Kinases Direct transfer of terminal phosphoryl group of ATP to substrate Creatine kinase Adenylate kinase Hexokinasc Phosphoglycerate kinase Pyruvate kinase Protein kinase Myokinase Phosphofructokinase Type 1 (M-S-E) Type 1 Type 1 Type 1 Type 2 (S-M-E)... 

A convenient synthesis of (,Sp)-ATPaS is the enzymatic phosphorylation of AMPS, adenosine 5 -phosphorothioate by the adenylate kinase-pyruvate kinase system according to Equation [23] ... 

The configuration of AMPS, lsO was shown to be (Rp), corresponding to inversion of configuration, by stereospecific phosphorylation, using the adenylate kinase-pyruvate kinase system, and analysis for bridging and nonbridging l80. Similar approaches have been applied to amino acyl-tRNA synthetases, which also catalyze inversion of P of ATP [79]. 

A similar conclusion was arrived at by Arnold and Pette ( 2) from studies carried out on the in vitro binding of aldolase glyceraldehyde phosphate dehyrogenase, fructose-6-phosphate kinase, phosphoglycerate kinase, pyruvate kinase and lactate dehydrogenase to the structural proteins F-actin, myosin, acto-myosin and stromaprotein. 

Hexosephosphate isomerase Phosphoglycerate kinase Pyruvate kinase Triosephosphate isomerase... 

Figure 16.21. Control of the Catalytic Activity of Pyruvate Kinase. Pyruvate kinase is regulated by allosteric effectors and covalent modification.

Phosphorylase kinase Glycogen synthetase Fructose-6-phosphate 2-kinase Pyruvate kinase Hormfure-sensitive lipase Aeetyl-CoA carboxylase... 

Enzyme pyruvate kinase. Pyruvate kinase is more active in the fed state than in the fasting state. 

Glycolysis (phosphoglycerate kinase, pyruvate kinase two ATPs are expended) 2... 

The reaction products, e.g. creatine, can be assayed by coupling the creatine kinase, pyruvate kinase, and LDH systems. The reduction of the absorbance of NADH is measured and equated to the concentration of creatinine. The enzymes must be rather pure, which makes the procedure very expensive. Since the physiological level of creatinine is as low as 100 pmol, the values obtained have to be corrected for pyruvate and creatine. 

Brovko Y, Romanova NA, Ugarova NN. Bioluminescent assay of bacterial intracellular AMP, ADP and ATP with the use of coimmobilised three-enzyme reagent (adenylate kinase, pyruvate kinase, firefly luciferase). Anal Biochem. 1994 220 410-4. 

The conditions given in Table 15.3 refer to the cell-free reaction mixture. In dialysis mode, this reaction mixture is dialyzed against a buffer of the same composition but lacking E. coli extract, RNA polymerase, creatine kinase/pyruvate kinase, and mRNA/DNA template. If a dialysis membrane with a molecular weight cut-off... 

The stereochemical experiment employing the Rp diastereomer of cyclic [ 0, 0]dAMP as substrate was reported in 1981 by Gerlt and co-workers (20). This substrate was also synthesized from a precursor phosphoroanilidate by reaction with C 02. The hydrolysis reaction was performed in H2 0 to generate one of the diastereomers of [ 0, 0, 0]dAMP as product. The configurational analysis of this material was performed with very high stereochemical integrity by first using adenylate kinase, pyruvate kinase, a trace of ATP, and... 

Creatinine can also be determined by measuring the rate of disappearance of NADH after addition of creatinine amidohydrolase, creatine kinase, pyruvate kinase, and lactate dehydrogenase. 

In the case of the enzymes glycerol kinase, pyruvate kinase and hexokinase inversion of configuration was observed at the chiral y-phosphorus (32). 

---