Kill tanks

XIII) Killing Tank at KOW contained, according to Ref 9, p 59, various waste liquids and slurries in which azides are likely to be present. When a sufficient quantity of such material was accumulated (not less than 1000 liters), the operator stirred the contents of the tank and took a 6 02 sample. If a small portion of the sample gave a positive test for azides (a red ppt of iron azide obtained on adding few drops of 1% feCl3 soln), a quantitative test was conducted as follows ... 

The "second mother liquor was analyzed for its NaN3 content and if it was over 2%, the liquor was reworked in the mother liquor evaporator. If the liquor contained less than 2% NaN3 it was sent to the 710 gal killing tank, where the azide was destroyed by a ealed amt of Na nitrate and coned sulfuric acid to produce the reaction ... 

After destroying the azide, the waste liquor of the killing tank was siphoned to a settling pond from which it was sent to a stream... 

Analysis of Ethyl Alcohol Solution A573-R Analysis of "Killing Tank Liquid A573 R Analysis of Nitric Acid Used for "Killing LA A574-L... 

Figure 3 shows a more detailed schematic of a TFF System in a cell harvesting application. In this particular set up, the system is designed for extracellular product processing but the principal components would also be used for intracellular products. It shows the plumbing of a clean-in-place system and a tank for collecting waste. The system is closed, in that all waste solutions existing the system enter a kill tank for sterilization. 

The main biosafety features of the fermentation system include the use of steam barriers on double O-ring seals, supply lines and mechanical seals on stirrer shafts, multiple O-ring seals, piping of condensate lines and pressure relief systems to a kill-tank , double (in series) filtration of inlet... 

---