Kappa-casein

Chowdhury P B and Luckham P F 1995 Interaction forces between kappa-casein adsorbed on mica Colloids Surfaces B 4 327-34... 

A useful property of the red seaweed extracts is their abiUty to form gels with water and milk. Kappa-carrageenan reacts with milk protein micelles, particularly kappa-casein micelles. The thickening effect of kappa-carrageenan in milk is 5—10 times greater than it is in water at a concentration of 0.025% in milk, a weak thixotropic gel is formed. 

Chabance B, JoUes P, Izquierdo C, Mazoyer E, Francoual C, Drouet L, Eiat AM. (1995) Characterization of an antithrombotic peptide from kappa-casein in newborn plasma after milk ingestion. Br J Nutr 73 583-590. 

Addeo, F., Chobert, J.-M. and Ribadeau-Dumas, B. 1977. Fractionation of whole casein on hydroxyapatite. Application of a study of buffalo kappa-casein. J. Dairy Res. 44, 63-68. 

Cheeseman, G. C. 1962. A method of preparation of kappa-casein and some observations on its nature. J. Dairy Res. 29, 163-171. 

Kim, Y. K., Yaguchi, M. and Rose, D. 1969. Isolation and amino-acid composition of para-kappa-casein. J. Dairy Sci. 52, 316-320. 

McKenzie, H. A. and Wake, R. G. 1961. An improved method for the isolation of kappa-casein. Biochim. Biophys. Acta 47, 240-242. 

Pujolle, J., Ribadeau-Dumas, B., Gamier, J. and Pion, R. 1966. A study of kappa-casein components. I. Preparation. Evidence of a common C-terminal sequence. Biochem. Biophys. Res. Commun. 25, 285-290. 

Saito, T., Itoh, T. and Adachi, S. 1979. Rapid preparation method of para-kappa-casein from whole casein. Jpn. J. Dairy Food Sci. 28, A183-A188. 

Schmidt, D. G. 1967. Fractionation of kappa-casein by column electrophoresis. Protides Biol Fluids 14, 671-676. 

Swaisgood, H. E. and Brunner, J. R. 1962. Characterization of kappa-casein obtained by fractionation with trichloracetic acid in a concentrated urea solution. J. Dairy Sci. 45, 1-11. 

Tripathi, K. K. and Gehrke, C. W. 1970. Chemical and chromatographic isolation of kappa-casein. J. Chromatography 46, 280-285. 

Vreeman, H. J., Both, P., Brinkhuis, J. A. and van der Spek, C. 1977. Purification and some physicochemical properties of bovine kappa-casein. Biochim. Biophys. Acta 491, 93-103. 

Stothart and Cebula (1982) and Stothart (1989) obtained linear Guinier plots of sub-micelles of whole casein, giving a radius of gyration of 64 A (Figure 1). On a uniform sphere model, this would indicate sphere radius of 84 A. This sphere size was input to a model fitting calculation (below). Thum et al (1987) found similar sizes for sub-micelles of kappa-casein. 

Tphe kinetics of the protease-triggered clotting of blood and milk has been formulated in a number of recent publications from this laboratory (1,2,3). In milk clotting, the coagulation is initiated through the limited proteolysis of -casein, the milk protein component which normally protects the casein micelles from flocculation by calcium ions (4). Kappa-casein is a single polypeptide chain of 169 residues, the sequence... 

Kappa-casein was prepared according to McKenzie and Wake (14). The protein was dissolved in 0.001 M imidazole buffer, pH 6.7, the ionic strength of which was adjusted by adding NaCl. 

Figure 6. Arrhenius plot for the clotting of K-casein brought about by rennet. Experimental conditions pH 6.7 I = 0.2 31.5°C. Kappa-casein concentration 0.1 g/100 mL clotting strength in reaction mixture, 3.97...

Chatchatee, R, Jarvinen, K.M., Bardina, L., Vila, L., Beyer, K., and Sampson, H.A. 2001b. Identification of IgE and IgG binding epitopes on beta- and kappa-casein in cow s milk allergic patients. Clin Exp Allergy 31(8) 1256—1262. 

Rasmussen, L.K., Hojrup, P., and Petersen, T.E. 1992. The multimeric structure and disulfidebonding pattern of bovine kappa-casein. Eur J Biochem 207(l) 215-222. 

Bal dit Sollier, C., Drouet, L., Pignaud, G., Chevallier, C., Caen, J., Fiat, A.M., Izquierdo, C., and Jolles, P. 1996. Effect of kappa-casein split peptides on platelet aggregation and on thrombus formation in the guinea-pig. Thromb. Res. 81, 427-437. 

Chymosin (calf and fermentation derived) Cleaves a single bond in kappa casein. 

Chymosin protease (1) Aspergillus niger var. awamori d-calf prochymosin gene (2) Escherichia coli K-12 d-calf prochymosin gene (3) Kluyveromyces marxianus cleaves a single bond in kappa casein cleaves a single bond in kappa casein 3.4.23.4... 

Practical Coacervate Formation. The composition of the various parts of the coacervate system was determined by using l c-labelled kappa-casein as one component of the coacervates, and then performing a mass balance on the system. The C-kappa-casein was prepared by reductive methylation of kappa-casein with l C-formaldehyde (15), This derivatization of kappa-casein was found not to materially affect its physical properties (16). 

To determine the composition of the coacervates, C-la-belled kappa-casein waS incorporated in the coacervates and a mass balance on the coacervate system was performed. The results of these studies are shown in Table III. Sixty percent of the kappa-casein in the gum arabic-kappa-casein coacervate system... 

Difficult to conclude how much kappa-casein is actually in the coacervates... 

Figure S. Photomicrographs of gum arabic/gelatin coacervate (left) and gum arabic/kappa-casein coacervate fright,), each at 470 X...

Some characteristics of kappa-casein/gum arabic coacervates using l C-labelled kappa-casein. 

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