K562 cells study

K562 CELLS STUDY WITH SCANNING FORCE AND CONFOCAL LASER SCANNING MICROSCOPY... 

The uptake of iron from transferrin into cells has been best studied for reticulocytes, where there is heavy demand for iron for the synthesis of heme. Reticulocytes and, by analogy, other mammalian cells have receptor molecules on their plasma surface which bind transferrin. Characterization of the transferrin receptor from rabbit reticulocytes suggests that it is probably a glycoprotein of molecular weight around 200 000. The number of receptor sites in cells varies in response to the conditions. For example, treatment of K562 cells with the efficient iron chelator desferrioxamine results in an increase in the total number of receptors for transferrin.1142... 

This study briefly introduces both the surface morphology features of K562 cells and their internal ultrastructure. U sing scanning force and confocal laser scarming microscopy allows us to visualize K562 cell surface morphology details such as the membrane convexities, different kind of surface microvilli, the plasma membrane surface... 

Scanning force microscopy (SFM) has been widely used for visualization of biomedical objects because of combination of extreme resolution, simplicity of sample preparation and ability to operate under physiological conditions. Nowadays SFM is increasingly applied to investigate the ultrastructure of biomedical samples embedded in epoxy resin [1]. In the present work, we are focusing on application of SFM, confocal laser scanning microscopy and ultramicrotomy to the K562 leukemic cells study. 

The aim of this study is to apply SFM combined with confocal laser scanning microscopy to investigate K562 cells surface morphology in details. Also an attempt was made to visualize with SFM the internal ultrastructure of embedded in epoxy resin of K562 leukemic cells. 

Ward, T. H., Butler J., Shahbakhi, H and Richards, J. T. (1997) Comet assay studies on the activation of two diaziridinylbenzoquinones in K562 cells. Biochem. Pharmacol. 53,1115-1121. 

Radosevic K, Schut TC, van Graft M, De Grooth BG, Greve J. A flow-cytometric study of the membrane potential of natural killer and K562 cells during the cytotoxic process. J Immunol Methods 1993 161(1) 119-128. 

NON possesses antitumor activity both against mammalian cell lines in vitro and against Crocker sarcoma 180 in studies with mice [277]. NON is also an inhibitor of the 170-kDa P-glycoprotein-mediated efflux of 4-(9 -tetrahydropyranyldoxorubicin in multidrug-resistant erythroleukemia K562 cells at subtoxic concentrations [278]. 

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