Isotope dilution mass spectrometry pantothenic acid

Stable isotope dilution mass spectrometry (IDMS) can be a definitive analytical method for very accurate concentration determination of pantothenic acid (Cornelis 2003). However, definitive accuracy of the IDMS technique requires full equilibration between endogenous natural amounts of the compound and the isotopically labelled spike, which is usually accomplished by bringing both in the same chemical form in solution. 

Isotope dilution mass spectrometry (IDMSO has been successfully used for pantothenic acid analysis (Chen et al. 2007 Rychlik 2003). IDMS is an important method that can acquire the most accurate value possible for the pantothenic acid concentration in a sample. However, with the release of the NIST SRM 3280, the accuracy of a method used can be determined easily. Since IDMS is very expensive, routine use of the method is not recommended due to the associated cost. 

There are no convenient or reliable functional tests of pantothenic acid status, thus assessment is made by direct measurement of whole blood or urine pantothenic acid concentrations. Urine measurements are perhaps the easiest to conduct and interpret, and concentrations are closely related to dietary intake, Whole blood measurements are preferred to plasma, which contains only free pantothenic acid and is insensitive to changes in pantothenic acid intake. Concentrations of pantothenic acid in aU of the above fluids can be measured by microbiological assay, most commonly using Lactobacillus plantarum. Whole blood must first be treated with an enzyme preparation to release pantothenic acid fi om CoA. Other techniques that have been used to measure pantothenic acid in human samples include radioimmunoassay and gas chromatography, Other techniques that have been developed include gas chromatography-mass spectrometry and a stable isotope dilution assay. CoA and AGP can be measured by enzymatic methods. ... 

Rychlik, M., 2003. Pantothenic acid quantification by a stable isotope dilution assay based on liquid chromatography-tandem mass spectrometry. Analyst. 128 832-837. 

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