Phosphate buffer, isotonic

Calf kidneys, dog kidneys and rhesus monkey kidneys were treated with trypsin to give suspensions of cells. The suspensions were centrifuged and the packed cells diluted with 400 volumes (calf cells) or 200 volumes (dog cells and rhesus monkey cells) of a growth medium consisting of 5% horse serum and 0.5% lactalbumen hydrolysate in Earle s saline, with 100 units/ml each of penicillin and streptomycin. These media were used separately to produce Semliki Forest/calf interferon, Semliki Forest/dog interferon and Semliki Forest/rhesus monkey interferon. The cell-containing growth medium was dispensed into 500 ml medical flat bottles (70 ml in each). The cultures were incubated at 36°C. Confluent sheets of cells (monolayers) were formed in 5 to 6 days. The growth medium was then removed and the monolayers were washed with isotonic phosphate-buffered saline, pH 7.5. 

We have included in this Section the presentation of aminoacyltriazenes since they can be formally regarded as imino derivatives of hydrazide. Aminoacyltriazenes have been developed as chemically stable triazene prodrugs capable of enzymatic hydrolysis under physiological conditions to liberate cytotoxic monomethyltriazene antitumor agents 4.276 [189], The aminoacyl-triazene prodrugs were found to undergo hydrolysis in isotonic phosphate buffer and in human plasma. A /3-alanyl derivative (4.274) was more stable... 

COMPUTER ALGORITHMS SOFTWARE Isothermal dilution-induced disassembly, DEPOLYMERIZATION, END-WISE ISOTHERMAL PROCESS ADIABATIC PROCESS ISOTONIC ISOTONIC BUFFERS Isotonic phosphate buffers,... 

When tetrahydrocannabinol in isotonic phosphate buffer was dialyzed against isotonic phosphate buffer, 50 - 100% of the drug was bound to the tubing used as the membrane. All of the drug was bound below 0.05 Ug/ml. Ultrafiltration was equally unsuccessful, since only 0 - 5% of the drug in isotonic phosphate buffer was recovered in the ultrafiltrate. 

Isotonic phosphate buffer (310mM sodium phosphate)... 

Add 32 ml of isotonic phosphate buffer to a number of 250-ml plastic centrifuge bottles. Add 180 ml of whole pig blood to each bottle, cap, and invert several times to mix. 

Gently resuspend the erythrocyte pellet from each bottle in 120 ml of isotonic phosphate buffer. Centrifuge at 1000 X g for 10 min and again remove the supernatant. 

Prior to the beginning of this experiment, the erythrocytes were washed twice in isotonic phosphate buffer. What was the purpose of these washes ... 

Isotonic phosphate buffer (310 mosM, pFI 6.8)—Dissolve 85.6 g of NaH2P04 (monobasic monohydrate) and 88.0 g of Na2HP04 (dibasic anhydrous) in 9.6 liters of distilled water. Chill the solution, adjust the pH to 6.8 with HC1, and bring the final volume to 10 liters with distilled water. 

Hypotonic phosphate buffer (20 mosM, pH 6.8)—Add 600 ml of isotonic phosphate buffer to 8.7 liters of distilled water. Adjust the pH to 6.8 if necessary. 

In order to illustrate the use of the lactoperoxidase procedure as a vectorial probe, the example to be given is the iodination of the plasma membrane of the human lymphocyte. All manipulations were carried out at 4°. The lymphocytes were isolated and washed free of extracellular protein in isotonic phosphate buffer. The cells were centrifuged at 700 g for 10 min and the supernatant serum was removed. Cells are resuspended in 5 to 10 volumes of phosphate buffer and centrifuged free of the supernatant. This procedure was repeated four times or more until the supernatant gave a 280 nm reading below an optical density of 0.1 absorbance. The pellet of washed cells were then suspended in 3 volumes of isotonic phosphate buffer to produce 2 x 10 cells/ml. One millicurie of I was added and 20 nmol of lactoperoxidase for each milliliter of cell suspension. Ten microliter portions of 1 mM peroxide was added at 1 min intervals. The lymphocytes were then spun down at 500 g for 5 min and washed three times with phosphate-buffered saline. 

Fig. 11 Hemolytic effects of CD derivatives on human erythrocytes in isotonic phosphate buffer (pH 7.4) at 37°C for 5 minutes. , P-CD , SBEl-P-CD , (2HP)3-P-CD A, (2HP)7-P-CD A, SBE4-P-CD O, SBE7-P-CD. (Adapted from Ref l)...

FIGURE 40.8 Release profiles of cholesterol from rabbit skin treated with p-CyDs (1.2mM) in isotonic phosphate buffer (pH 7.4) at 37°C. O, Control , p-CyD , DM-P-CyD. Each point represents the mean SE of 12 experiments. 

Receptor fluids described in the literature range from water alone to isotonic phosphate buffers containing albumin, which increases solubility (Dick et al. 1996). Microbial growth can produce problems due to partitioning of the permeant into, or metabolism of the... 

Sample preparation Inject a 10 p.L aliquot of dialysate (pH 7.4 isotonic phosphate buffer). 

Assay of Protein Synthesis. For radiolabeling, H9 cells (3 x 10 cells per well in microtiter plates) were grown in the presence of ascorbate at 0, 75, 100, and 150 ig/ml as described earlier. On days 1, 2, and 4, cells were harvested, washed, and resuspended in methionine- and cysteine-free medium and then incubated at 37°C for 30 min in 0.5 ml of the same medium supplemented with 50 juCi of Tran S-label (specific activity, 1013 Ci/mmol ICN), Labeled cells were pelleted, washed in isotonic phosphate-buffered saline, resuspended in lysis buffer containing 1% Nonidet P-40, and... 

Sub-microscopic glass particles (most frequent diameter <0.1 pm) suspended in an electrolyte solution were capable of acting on developing synthetic lipid membranes or pre-formed bilayer lipid membranes by achieving contact with the surface of such membranes and finally entering them (Majer 1971). Rat erythrocytes pre-treated with lipophilic ethyl-3,5,6-tri-0-benzyl d-glucofuranoside emulsified in isotonic phosphate buffer were protected from haemolysis by glass particles in a dose-related manner (Majer 1975). 

A 2.0% w/v solution of pilocarpine nitrate in pH 5.5 isotonic phosphate buffer (hereafter referred to as S), and a viscous gel containing 1.54% w/v pilocarpine base and 0.77% w/v poly(acrylic acid)... 

Results of initial voltammetric studies on washed suspensions of intact human RBC in a pH 7.4 isotonic phosphate buffer suggested that at a dropping mercury electrode (D.M.E.) there was an interaction between the electrode and the sulfhydryl groups associated with the membrane. This conclusion was based on the findings that the observed wave -0.220 V versus S.C.E.) was not present... 

Locust bean gum i-carrageenan microparticles Gentamicin Gel having gentamicin encapsulating by gum microparticles In-vitro experiments in isotonic phosphate buffer solution (pH 7-4) at 37°C., showed that the release of gentamicin sulphate was dependent on concentration of LBG. [149]... 

---