Isothermal titration calorimetry interactions

T. K. Dam and C. F. Brewer, Thermodynamic studies of lectin-carbohydrate interactions by isothermal titration calorimetry, Chem. Rev., 102 (2002) 387 -29. 

Further experiments focused therefore on [RuCl(en)(r 6-tha)]+ (12) and [RuCl(rj6-p-cym)(en)]+ (22), which represent the two different classes, and their conformational distortion of short oligonucleotide duplexes. Chemical probes demonstrated that the induced distortion extended over at least seven base pairs for [RuCl(rj6-p-cym)(en)]+ (22), whereas the distortion was less extensive for [RuCl(en)(rj6-tha)]+ (12). Isothermal titration calorimetry also showed that the thermodynamic destabilization of the duplex was more pronounced for [RuCl(r 6-p-cym)(en)]+ (22) (89). DNA polymerization was markedly more strongly inhibited by the monofunctional Ru(II) adducts than by monofunctional Pt(II) compounds. The lack of recognition of the DNA monofunctional adducts by HMGB1, an interaction that shields cisplatin-DNA adducts from repair, points to a different mechanism of antitumor activity for the ruthenium-arenes. DNA repair activity by a repair-proficient HeLa cell-free extract (CFE) showed a considerably lower level of damage-induced DNA repair synthesis (about six times) for [RuCl(en)(rj6-tha)] + compared to cisplatin. This enhanced persistence of the adduct is consistent with the higher cytotoxicity of this compound (89). 

Christensen, T., B. Svensson, and B.W. Sigurskjold. 1999. Thermodynamics of reversible and irreversible unfolding and domain interactions of glucoamylase from Aspergillus niger studied by differential scanning and isothermal titration calorimetry. Biochemistry 38 6300-6310. 

Association Constants (K ) for Interaction Between ConA and Selected Monosaccharides by Isothermal Titration Calorimetry... 

The enthalpy change associated with formation of a thermodynamically ideal solution is equal to zero. Therefore any heat change measured in a mixing calorimetry experiment is a direct indicator of the interactions in the system (Prigogine and Defay, 1954). For a simple biopolymer solution, calorimetric measurements can be conveniently made using titra-tion/flow calorimeter equipment. For example, from isothermal titration calorimetry of solutions of bovine P-casein, Portnaya et al. (2006) have determined the association behaviour, the critical micelle concentration (CMC), and the enthalpy of (de)micellization. 

McClements, D.J. (2000). Isothermal titration calorimetry study of pectin-ionic surfactant interactions. Journal of Agricultural andFood Chemistry, 48, 5604-5611. 

Wangsakan, A., Chinachoti, P., McClements, D.J. (2001). Maltodextrin-anionic surfactant interactions isothermal titration calorimetry and surface tension study. Journal of Agricultural and Food Chemistry, 49, 5039-5045. 

Application of Isothermal Titration Calorimetry to Thermodynamic Studies of Biomolecular Interactions. 

The traditional methods of evaluating the interactions of glycans and glycan-binding proteins are based on isothermal titration calorimetry, surface plasmon resonance (SPR), and enzyme-linked lectin assays. Recently, glycan arrays have been developed as a... 

Fig. 12.12 Top Interaction of 4e with human serum albumin as probed by isothermal titration calorimetry, a single-site model yielded a stoichiometry of 5 1 of 4e HSA with a kn of 2 pM. Middle Inhibition of hemolysis of 4e by HSA. Bottom Identical potency of NO inhibition of 4e solubilized in DMSO or in HSA...

TRAP recruitment requires Hgand-induced changes in receptors that allow simultaneous interactions of each TRAP trimer with three receptor intracellular domains. This observation implicates that monomeric TRAF-receptor interactions are of low affinity so that the interactions do not occur in the absence of receptor activation. A number of quantitative biophysical characterizations with isothermal titration calorimetry (ITC) and surface plasma resonance (SPR) have provided solid support to this view (Table III). 

Ye, H., and Wu, H. (2000). Thermodynamic characterization of the interaction between TRAF2 and receptor peptides by isothermal titration calorimetry. PNAS 97, 8961-8966. 

Pierce, M. M., Raman, C. S., Nall, B. T. (1999). Isothermal titration calorimetry of protein-protein interactions. Methods 19, 213-221. 

Poncet-Legrand, C., Gautier, C., Cheynier, V, Imberty, A. (2007). Interactions between flavan-3-ols and poly(L-prohne) studied by isothermal titration calorimetry Effect of the tannin structure./. Agric. Food Chem., 55, 9235-9240. 

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