Isolation of microorganisms

When an aliquot of grape must or wine is placed on a suitable solidified medium, colonies of various morphologies will appear on the plate. In theory, a single viable cell in the original sample continuously multiplies to eventually yield one visible colony after a suitable incubation. 

Hold the transfer loop in the right hand and flame the loop to red-hot in an open flame. Cool the loop by carefully touching the surface of 

Using the same procedures as described in Section 13.8, remove a colony (or a mass of colonies) and place the loop at position 1 shown in Fig. 13.3. 

Carefully move the loop across the agar surface following the 1 pattern ( zig-zag ). The best separation is effected when streaks are kept as tight as possible and the maximum surface area of the plate is utilized. Avoid digging into the agar with the loop. 

When 1 pattern is completed, replace the Petri plate lid, and flame the loop to sterilize. 

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Isolation of microorganisms having aldoxime dehydratase activity ... 

As described above, the procedure and some of our examples of successful isolation of microorganisms having new enzymes were briefly described. Furthermore, historic changes of the way and methods of the research on the degradation of nitrile compounds and the exploitation of some industrially important enzymes including NHase, etc., were introduced. 

Aislabie, J. Rothenburger, S., and Atlas, R. M., Isolation of Microorganisms Capable of Degrading Isoquinoline under Aerobic Conditions. Applied Environ. Microbio, 1989. 55(12) pp. 3247-3249. 

Secondary intraabdominal infections are often polymicrobial. The mean number of isolates of microorganisms from infected intraabdominal sites has ranged from 2.9 to 3.7, including an average of 1.3 to 1.6 aerobes and 1.7 to 2.1 anaerobes. The frequencies with which specific bacteria were isolated in intraabdominal infections are given in Table 42-2. 

Kurane, R., Suzuki, T., and Takahara, Y. Microbial degradation of phthalate esters. 1. Isolation of microorganisms growing on phthalate esters and degradation of phthalateester by Pseudomonas acidoForans 256-1, Agric. Biol Chem., 41(11) 2119-2123, 1977. 

Lopez, C.E. and Kirkwood, J.I. Isolation of microorganisms from a Texas soil capable of degrading nrea derivative herbicides. Soil Sci. Soc. Am. Proa, 38 309-312,1974. 

Based on the rate at which novel microorganisms are being discovered, one can make an estimation of the amount of different organisms that have been identified up to now relative those that remain uncharacterized (Table 5.8). The overwhelming biochemical diversity present in nature makes that the isolation of microorganisms with novel enzymatic activities is worthwhile (reviewed in Bull, Goodfellow Howard Slater, 1992). Preferred sites to find novel enzymes include so-called mega-diversity countries such as Mexico, Colombia, Brazil or India and biodiversity hot spots like rain forest and deep seas. 

Yoshiki (29) has studied the biosynthetic pathways of vitamin Bg, including the discovery of the precursor glycolaldehyde, glycolaldehyde dehydrogenase, and isolation of microorganisms deficient in regulation mechanism of Bg biosynthesis. 

N. Suresh, R. Warburg, M. Timmerman, J. Wells, M. Coccia, M. F. Roberts and H. O. Halvorson (1985). New strategies for the isolation of microorganisms responsible for phosphate accumulation. 

Isolation of microorganisms from environments which correlate with the educts. 

Lynch, J. (1972) Identification of substrates and isolation of microorganisms responsible for ethylene... 

Table V Isolation of microorganisms from lily roots treated with antagonistic bacteria...

Pouton CW, Majid MIA, Notarianni LJ (1988) Degradation of polyhydroxybutyrate and related copolymers. Proc Int Symp Control Release Bioact Mater 15 182-183 Ramsay BA, Saracovan I, Ramsay JA, Marchessault RH (1994) A method for the isolation of microorganism producing extracellular long-side-chain poly(P-hydroxyalkanoate) depolymerase). J Environ Polym Degrad 2 1-7... 

GUSMAO D S, SANTOS, A V, MARINI D C, RUSSO E S, PEKOTO AMD, JUNIOR M C, BERBERT-MOLiNA M A and LEMOS F J A (2007) First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera Cuhcidae) new prespectives for an insect-bacteria association, Mem Inst Oswaldo Cruz, 102, 919-924. 

Table 5 Isolation of microorganisms from exterior coatings in Germany...

Hass H, Herfiirth E, Stoffler G, Redl B (1992) Purification, characterization and partial amino acid sequences of a xylanase produced by Penicillium chrysogenum. Biochim Biophys Acta 1117 279-286 Heisey RM, Papadatos S (1995) Isolation of microorganisms able to metabolize purified natural rubber. Appl Environ Microbiol 61 3092-3097 Hernandez G, Kucera V, Thierry D, Pedersen A, Hermansson M (1994) Corrosion inhibition of steel by bacteria. Corrosion 50 603-608 HespeU RB, O Bryan-Shah PJ (1988) Esterase activities in Butyrivihrio fibrisolvens strains. Appl Environ Microbiol 54 1917-1922 HiU EC (1987) Microbial problems in the off-shore oil industry. Institute of Petroleum, London, pp 25-28... 

Heisey, R.M., and Papadatos, S., 1995, Isolation of microorganisms able to metabolize purified natural rubber. Appl. Environ. Microbiol. 61 3092-3097. 

Ramsay et al. [179] developed a method for the specific isolation of microorganisms producing extracellular depolymerases for medium-side-chain PHAs. A sterile, colloidal suspension of a copolyester of 3HB, 3HHx, 3HO and 3HD monomers was used as enrichment medium, and six bacterial species capable of growing in it were isolated. Not surprisingly, all were identified as pseudomonads or members of related genera. 

Hong K, Chen GQ, Tian WD, Huang WY and Fan QS, Isolation of microorganisms capable of synthesizing novel biopolymers from oil contaminated soil and water. 

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