Iron sensing

In response to iron deprivation, E. chrysanthemi induces the synthesis of siderophores and also the transcription of pectate lyase genes (52). The pelB, pelC, pelE and pelL genes are induced under Umited iron-deprivation whereas pelD is only induced under severe iron-deprivation (53). Iron sensing in E. 

Sumner JP, Kopelman R (2005) Alexa Fluor 488 as an iron sensing molecule and its application in PEBBLE nanosensors. Analyst 130 528-533... 

Van Tamelen 19 proposed that if such a mechanism is operative, one might expect the conversion of cyclopenteno oxide (lie) inlo cyclopentene sulfide (Via) (Eq. 2) to be difficult since it would involve a strained intermediate (IVo possessing two five-membered rings fuscil in a Irons sense. Experimentally, treatment of (lie) with potSHriuni... 

The iron-sulfur center at the active site of the IRE-BP is rather unstable, and loss of the iron triggers significant changes in protein conformation. Thus, this protein can serve as an iron-sensing factor. 

FIGURE 22.3 Model for Uver-centred serum iron sensing. (From Schmidt, Toran, Giannetti, Bjorkman, Andrews, 2008. Copyright 2008 with permission from Elsevier.)... 

Outline the role of RNA secondary structure in the regulation of iron metabolism in animals. Describe the roles of transferrin, transferrin receptor, ferritin, the iron-response element, and IRE-binding protein. Relate the IRE-binding protein to aconitase and iron sensing. 

At contact fatigue tests of different steel and cast iron types was used the acoustic emission technique. Processed records from the AE analyser show importance of acoustic response of tested surface continuous sensing. In graphs are obvious characteristic types of summation curves, or may be from significant changes of AE signal course identified even phases of the wear process. 

For organometailic compounds, the situation becomes even more complicated because the presence of elements such as platinum, iron, and copper introduces more complex isotopic patterns. In a very general sense, for inorganic chemistry, as atomic number increases, the number of isotopes occurring naturally for any one element can increase considerably. An element of small atomic number, lithium, has only two natural isotopes, but tin has ten, xenon has nine, and mercury has seven isotopes. This general phenomenon should be approached with caution because, for example, yttrium of atomic mass 89 is monoisotopic, and iridium has just two natural isotopes at masses 191 and 193. Nevertheless, the occurrence and variation in patterns of multi-isotopic elements often make their mass spectrometric identification easy, as depicted for the cases of dimethylmercury and dimethylplatinum in Figure 47.4. 

Silicates. For many years, siUcates have been used to inhibit aqueous corrosion, particularly in potable water systems. Probably due to the complexity of siUcate chemistry, their mechanism of inhibition has not yet been firmly estabUshed. They are nonoxidizing and require oxygen to inhibit corrosion, so they are not passivators in the classical sense. Yet they do not form visible precipitates on the metal surface. They appear to inhibit by an adsorption mechanism. It is thought that siUca and iron corrosion products interact. However, recent work indicates that this interaction may not be necessary. SiUcates are slow-acting inhibitors in some cases, 2 or 3 weeks may be required to estabUsh protection fully. It is beheved that the polysiUcate ions or coUoidal siUca are the active species and these are formed slowly from monosilicic acid, which is the predorninant species in water at the pH levels maintained in cooling systems. 

The author has often wondered how often machines are redesigned and rebuilt or even replaced for reasons that were later found to be incorrect. Based on observations over a considerable number of years, the number might be staggering. Ironically, the incorrect diagnosis sometimes results in a solution that will at least for the time being appear to fix the problem. This type of solution may possibly lead to a future problem because an incorrect symptom-cause relationship is established that will not hold true on another application at another time. All of this is being said to stress the need for proper and careful problem solving that accurately determines the real cause for the problem. It is difficult to address the common sense side of reliability and not make some type of... 

Wood is particularly valuable for many conditions which are corrosive to common metals (e.g. acids and external exposure), and for contact with foodstuffs and beverages. It is not subject to corrosion in the electrochemical sense of the term applied to metals, but in saline conditions it can be attacked by the products of metal corrosion (alkali and iron salts) where poor technology or unsuitable wood species are used. Although wood is attacked by both extremely alkaline and acid conditions, particularly those which are oxidising, it can be employed over a wider pH range than most other materials. 

Structural types for organometallic rhodium and iridium porphyrins mostly comprise five- or six-coordinate complexes (Por)M(R) or (Por)M(R)(L), where R is a (T-bonded alkyl, aryl, or other organic fragment, and Lisa neutral donor. Most examples contain rhodium, and the chemistry of the corresponding iridium porphyrins is much more scarce. The classical methods of preparation of these complexes involves either reaction of Rh(III) halides Rh(Por)X with organolithium or Grignard reagents, or reaction of Rh(I) anions [Rh(Por)] with alkyl or aryl halides. In this sense the chemistry parallels that of iron and cobalt porphyrins. 

Many types of oxide layers have a certain, not very high electrical conductivity of up to 10 to 10 S/cm. Conduction may be cationic (by ions) or anionic (by or OH ions), or of the mixed ionic and electronic type. Often, charge transport occurs by a semiconductor hole-type mechanism, hence, oxides with ionic and ionic-hole conduction are distinguished (in the same sense as p-type and n-type conduction in the case of semiconductors, but here with anions or cations instead of the electrons, and the corresponding ionic vacancies instead of the electron holes). Electronic conduction is found for the oxide layers on iron group metals and on chromium. 

The spin state of a paramagnetic system with total spin S wiU lift its (25 + l)-fold degeneracy under the influence of ligand fields (zero-field interaction) and applied fields (Zeeman interaction). The magnetic hyperfine field sensed by the iron nuclei is different for the 25 + 1 spin states in magnitude and direction. Therefore, the absorption pattern of a particular iron nucleus for the incoming synchrotron radiation and consequently, the coherently scattered forward radiation depends on how the electronic states are occupied at a certain temperature. 

Do these prokaryotic organisms display a primitive iron metabolism, in the sense that they form a relatively limited number of iron compounds, and are they accordingly good candidates to suspect as direct descendants of the primordial cell ... 

Nanocrystalline gamma iron oxide (y-Fe203) recently been studied as a gas sensing material, has been synthesised at 70°C using sonication-assisted precipitation technique [21]. The synthesised material was then used for fabricating the... 

Another potential source of iron, at least for hepatocytes, is receptor-independent uptake of iron from transferrin. This appears to involve an iron uptake pathway from transferrin which is neither suppressed in hepatocytes by antibodies to TfR (Trinder et at, 1988), nor by transfection of HuH-7 hepatoma cells with transferrin receptor anti-sense cDNA (Trinder etat, 1996). The same pathway may also be utilized for iron uptake from isolated transferrin N-lobe, which is not recognized by the receptor (Thorstensen et at, 1995). The possible role of TfR2 in this process remains to be established, as does the physiological importance of this pathway in intact liver. Human melanoma cells (Richardson and Baker, 1994) and Chinese hamster cells lacking transferrin receptors but transfected with melanotransferrin (Kennard et at, 1995) use another pathway for transferrin iron uptake, independent of the transferrin receptor, but utilizing iron transfer from transferrin or simple iron chelates to membrane-anchored melanotransferrin, and from there onwards into the cellular interior. 

Figure 8.2 Rat duodenal cells divide in the crypts of Lieberktihn and differentiate while migrating to the villus tips within approximately 48 h. The crypt cells take up iron from the blood, and are thereby able to sense the body s state of iron repletion. They migrate to the villus tips where this information determines their iron absorption capacity from the intestinal lumen. Adapted from Schumann et al., 1999, by permission of Blackwell Science.

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