Involvement of Multiple Factors in Hatching Stimulation for Cyst Nematodes

Involvement of Multiple Factors in Hatching Stimulation for Cyst Nematodes 

The tomato root diffusate obtained from a hydroponic culture medium was concentrated with a rotary evaporator to give solid residue and concentrated aqueous extract. A vacuum line was connected to two traps in series trap I (-45 °C) and trap II (-196 °C). The water condensed from the rotary evaporator in trap I gave a pair of synergists, which were referred to as lA and IB (SylA and SylB). The highly volatile synergist was obtained in the trap, and was referred to as synergist II (Syll). 

On the other hand, the residue after the concentration by rotary evaporator was separated on normal phase gel adsorbents HP-20, Sephadex LH-20, and Toyopearl HW-40, to give 3 mg of a hatching stimulator. This material was active at 10 g/mL. The activity of this stimulant was increased more than 100-fold by SylA and SylB. Syll had a striking synergistic activity, enhancing the hatching activity ca. 100,000-fold. 

Based on NMR spectra, the hatching stimulator is tentatively concluded to be a triterpene glycoside, whereas SylA and IB are both presumed to diesters, and Syll to be a monoester. Detailed structure analyses are in progress. 

Baunacke, Arh. Biol. Bund Anst. Land und Forstw., 1922, 11,185-288. 

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