Intercellular adhesion molecule cell culture

Cell cultures expressing intracellular aptamers show reduced binding to Intercellular Adhesion Molecule I (ICAM-I). 

McCramick CJ, Craig A, Roberts D, Newbold Cl, Berendt AR Intercellular adhesion molecule-1 and CD36 syner e to mediate adherence of Plasmodium folciparum-infected erythrocytes to cultured human microvascular endothelial cells. J Clin Invest 100 2521-2529,1997... 

Intercellular adhesion molecule-1 (ICAM-1, CD54) and ICAM-2 (CD 102) are constitutionally expressed on cultured endothelial cells and both serve as ligands for CDlla/CD18.40-41 ICAM-1 possesses five extracellular Ig domains 42 the first and third are recognised by CD 11a and CD lib, respectively. Because ICAM-2 only has two Ig domains it binds CD 11a but not CD lib. The endothelial expression of ICAM-1... 

Expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) is known to be elevated at sites of inflammation. Studies have been conducted into the effects of EGCG and TF-3 on the expression of these adhesion molecules induced by interleukin-ip (IL-lp) in cultured human umbilical vein endothelial cells (HUVECs). Both compounds significantly inhibited IL-ip-induced protein expression of VCAM and ICAM in dose-dependent manners and were associated with reduced adhesion of leukocytes to HUVECs. The m-RNA level of VCAM-1 was also inhibited by these tea polyphenolics, as was the NF-KB-dependent transcriptional activity induced by IL-lp. It is concluded that these molecules exhibit anti-inflammatory and anti-invasion properties, probably via a route involving blockage of IkB kinase. 

Nagel, T., Resnick, N., Atkinson, W. J., Dewey, C. F. J., and Gimbrone, M. A. J. (1994). Shear stress selectively upregulates intercellular adhesion molecule-1 expression in cultured human vascular endothelial cells. /. Clin. Invest. 94, 885-891. 

Silica seems to be a potent activator of endothelial cells in vitro, too. Incubation of human dermal microvascular endothelial cells (HDMEC) with silica at non-toxic concentrations increased the steady-state levels of the messenger RNA (mRNA) for intercellular adhesion molecule 1 (ICAM-i), and also increased the corresponding levels of this cell-surface protein as shown by fluorescence-activated cell-sorter (FACS) analysis the incubation also increased the level of soluble protein in the culture fluid, as shown by means of ELISA, in a dose- and time-dependent manner. Additionally, increased levels of IL-6 in the culture supernatants have been found. In addition, a significant increase in coUagenase I mRNA in HDMEC has been demonstrated (Anderegg et al. 1997). 

Adamson lYR, Letourneau HL, Bowden DH (1989) Enhanced macrophage fibroblast interactions in the pulmonary inter-stitium increases fibrosis after silica injection to monocyte-depleted mice. Am J Pathol 134 411-418 American Thoracic Society (1997) Adverse effects of crystalline silica exposure. Am J Respir Grit Care Med 155 761-765 Anderegg U, Vorberg S, Herrmann K, Haustein UP (1996) Increased expression of interstitial collagenase in silica-treated fibroblasts. Eur J Dermatol 6 51-55 Anderegg U, Vorberg S, Herrmann K, Haustein UF (1997) Silica directly induces intercellular adhesion molecule 1 (ICAM-i) expression in cultured endothelial cells. Eur J Dermatol... 

Cell adhesion molecule gene expression is upre-gulated in ischaemia/reperfusion. Kacimi etal. (1998) studied intercellular adhesion molecule (ICAM) response to cytokines and acute hypoxia in cultured myocardial cells. Northern blot analysis and immunoassay showed that the proinflammat-ory cytokines interleukin-ip and tumour necrosis factor-a stimulated concentration dependent in-... 

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