Intercalation binding

In addition to UV binding studies of BPDE, one other less carcinogenic epoxide, ( ) trans-9,10-dihydroxy-anti-7,8-epoxy-7,8,9,10-tetrahydro-BP, was examined. Table IV which contains results obtained in this study indicates that the intercalation binding constant is 23% lower than that of BPDE (3 ). 

Table IV. Comparison of Results from UV Absorption Studies of the Intercalation Binding Constants for Benzo[a]pyrene Metabolites and Metabolite Model Compounds into DNA...

This relationship is useful for understanding the different intercalation binding constants of hydrocarbons with similar ir systems. This is indicated in Figure 4, by a comparison of data for 1,2,3,4-tetrahydro-BA, DMA and anthracene. For 1,2,3,4-tetra-hydro-BA the presence of a nonplanar alicyclic group is expected to sterically inhibit intercalation. The nonplanar methyl groups of DMA play a similar steric role. However for both 1,2,3,4-te-... 

Intercalation binding as a molecular mechanism of R factor elimination has been proposed by Hahn and Ciak68. Based on earlier data26-28, that tilorone intercalates... 

Major groove intercalators bind DNA with high affinity (Ka > 10 M- )and, in some cases, high sequence specificity. Indeed, an extended aromatic system on the ligand outward from the metal center, as in the case of the phi (9,10-phenanthrenequinone... 

Intercalator binding. Put an aliquot (20 pL) of the intercalator solution onto the SPE surface (Fig. 6A4, B4). Then, incubate for 5 min at room temperature. Wash off the excess intercalator with blank PBS. 

Apart from TMPyP4 and its derivatives, several other types of molecules could also interact with i-motif DNA to stabilize the structure or induce the formation of i-motif DNA. However, most of them interact with i-motif DNA through the end-stacking mode other modes are barely reported. In the case of i-motif DNA, intercalation binding is even scarcer than with G-quadruplexes because the size of the planar layer is small and alternating, and n-n stacking interaction is less possible. In addition, the distance between the base pairs layer is about 3.1 A, which is smaller than B-DNA. Much more work is required, however, to evaluate this possibility. 

The intercalation binding of Quinacrine with DNA double helix can be expressed by the equation ... 

It has been reported that intercalation binding of quinacrine has maximum saturation at one bound molecule per two base pairs (25). In other words, after one site is occupied, binding at neighboring site is excluded. So at saturation of binding, the concentration of the occupied site should be the same as that of the free site. 

DNA intercalation binding to single stranded poly (rA) inhibitor of topoisomerase I and II inhibition of DNA potymerase, reverse transcriptase... 

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