Interactome/interactomics

Interactomics (Interactome) The study of the interactions between genes, RNA, proteins and metabolites within the cell. 

Interactome Interactome is defined as the whole set of molecular interactions in cells. Databases describing molecular interactions and genetic networks will provide a link at the functional level between the genome, the proteome, and the transcriptome worlds of different organisms. Interaction databases therefore aim at describing the contents, structure, function, and behavior of what we herein define as the interactome world. 

Jalan V, Taylor EJ. 1983. Importance of interactomic spacing in catalytic reduction of oxygen in phosphoric acid. J Electrochem Soc 130 2299-2301. 

Ito, T., Chiba, T., Ozawa, R., Yoshida, M., Hattori, M., and Sakaki, Y. (2001). A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc. Natl. Acad. Sci. USA 98, 4569-4574. 

Figure 28.1 A small segment of the yeast interactome. The spheres represent proteins and the interconnecting lines are identified protein interactions. Many proteins are seen to interact with one or two other proteins, but some can have over a dozen other interacting partners.

Name Species Tissue specificity Function Interactome... 

Chua HN, Wong L (2008) Increasing the reliability of protein interactomes. Drug Discov Today 13 652-658... 

Gandhi, T.K., Zhong, J., Mathivanan, S., Karthick, L., Chandrika, K.N., Mohan, S.S., Sharma, S., Pinkert, S., Nagaraju, S., Perias-wamy, B., et al, (2006) Analysis of the Human Protein Interactome and Comparison with Yeast, Worm and Fly Interaction Datasets. Nat Genet, 38, 285-293. 

Chaurasia, G., Iqbal, Y., Hanig, C., Herzel, H., Wanker, E.E. and Futschik, M.E. (2007) Uniin An Entry Gate to the Human Protein Interactome. Nucleic Acids Res, 35, D590-594. 

Ge H, Liu, Z, Church GM et al. Correlation between transcriptome and interactome mapping data from Saccharomyces cerevisiae. Nature Genet 2001 29 482-486. 

Outcome of the methods used in bioinformatics allow scientists to build a global protein structural interaction map. The first developed map is called PSIMAP (Protein Structural Interactome Map). It has low resolution and allows production of a draft map for very large-scale protein interaction study. Protein maps reveal that protein structures have distinct preferences for their interacting partners and the interactions are not random. Some proteins have only one interaction partner whereas some have more. Some protein groups function as separately while others work within larger complexes. Also, many proteins possess homointeraction. 

Fig. 11. Molecular structure of (PhjPAu)3V(CO)5 phenyl groups have been omitted for clarity. Selected mean interactomic distances (A) V-Au, 2.73(2) Au-Au, 2.82(3) V-C, 1.95(4) C-0,1.16(2).

Ribas C, Penela P, Murga C, Salcedo A, Garcfa-Hoz C, Jurado-Pueyo M, Aymerich I, Mayor F Jr (2007) The G protein-coupled receptor kinase (GRK) interactome role of GRKs in GPCR regulation and signaling. Biochim Biophys Acta 1768(4) 913-922... 

Ito T, Ota K, Kubota H et al (2002) Roles for the two-hybrid system in exploration of the yeast protein interactome. Mol Cell Proteomics 1 561-566... 

The spectrum of potential dysbindin-1 functions is indicated by the types of proteins in its interactome. If we include both the known and candidate binding partners, the interactome includes ten protein groups... 

Camargo LM, Collura V, Rain J-C, Mizuguchi K, Hermjakob H, et al. 2007. Disrupted in schizophrenia 1 interactome Evidence for the close connectivity of risk genes and a potential synaptic basis for schizophrenia. Mol Psychiatry... 

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