DNA hybridization analysis

Qian KJ, Zhang L, Yang ML, He PG, Fang YZ (2004) Preparation of luminol-doped nanoparticle and its application in DNA hybridization analysis. Chinese J Chem 22 702-707... 

Heated electrodes are useful as chemical sensors, preferably in bioanalytical applications like DNA hybridization analysis. Other applications are kinetic studies using the temperature pulse method, and determination of physicochemical constants like redox entropy, etc. 

Samples from the MTBE-contaminated sites (Table 2) were screened by PGR and DNA/DNA hybridization analysis for the presence of the genes involved in the MTBE metabolic pathway using the primers presented in Table 4. 

Microfluidic DNA hybridization assays Nucleic acid hybridization assays On-chip DNA hybridization analysis... 

Ben-Yoav H, Dykstra PH, Bentley WE, Ghodssi R (2012) A microfluidic-based electrochemical biochip for label-free diffiision-restricted DNA hybridization analysis. Biosens Bioelectron 38 114—120... 

Ben-Yoav, H., Dykstra, P.H., Bentley, W.E., Ghodssi, R., 2015. A controlled microfluidic electrochemical lab-on-a-chip for label-free diffusion-restricted DNA hybridization analysis. Biosensors and Bioelectronics 64, 579-585. 

Functionalized CNTs are promising materials for the development of elearochemical DNA hybridization biosensors as well. In 2005, a DNA sensor was developed from a composite of PPy and CNTs, which were functionalized with carboxylic groups to covalently immobilize DNA. ° In another example, PPy films doped with CNTs functionalized with oligonudeotides were successfully implemented into DNA biosensors, which offers great promise for reagentless DNA hybridization analysis using direa... 

Fig. 6. DNA sequence analysis, (a) Simplified methodology for dideoxy sequencing. A primer, 5 -TCTA, hybridized to the template, is used to initiate synthesis by DNA polymerase, (b) Stmcture of 2, 3 -dideoxy CTP. When no 3 -OH functionaUty is available to support addition of another nucleotide to the growing chain, synthesis terminates once this residue is incorporated into the synthetic reaction, (c) Representation of a DNA sequencing gel and the sequence, read from bottom to the top of the gel, gives sequence information in the conventional 5 to 3 direction.

Freeman WM et al. Fundamentals of DNA hybridization arrays for gene expression analysis. BioTechniques 2000 29 1042-1055. 

Klein CA, Schmidt-Kittler O, Schardt JA, et al. Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells. Proc. Natl. Acad. Sci. USA 1999 96 4494-4499. 

Host-cell and vector-derived DNA. Residual DNA is detected by hybridization analysis, using suitably sensitive sequence-independent analytical techniques or other suitably sensitive analytical techniques. 

The intercalation of polycyclic aromatic compounds into duplex DNA structures was used to develop nucleic acid-based electrochemical sensors.66 For example, the bis-ferrocene-tethered naphthalene diimide (16) was used as a redox-active intercalator to probe DNA hybridization.67 The thiolated probe was assembled on a Au electrode, and the formation of the duplex DNA with the complementary analyte nucleic acid was probed by the intercalation of (16) into the double-stranded nucleic acid structure and by following the voltammetric response of the ferrocene units (Fig. 12.17a). The method enabled the analysis of the target DNA with a sensitivity that corresponded to ca. 1 x 10-20mol. 

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