Integrated PCR-CE microdevice

Figure 8.23. Real-time analysis of a / -globin PCR amplification using an integrated PCR-CE microdevice. Chip CE separations of the same sample were performed sequentially in the integrated PCR-CE microdevice after (a) 15, (b) 20, (c) 25, and (d) 30 cycles at 96°C for 30 seconds and 60°C for 30 seconds. (Reprinted with permission from Ref. 47.)...

Integrated Microdevices for Bioiogicai Appiications, Fig. 6 Schematic of a fully integrated PCR-CE microdevice. The device consists of microfabricated heaters, RTDs, 8 parallel PCR chambers, and a CE device (Reprinted with permission from Lagally et al. [9])... 

Schematic drawing of the integrated PCR-CE microdevice (a) Laser-excited confocai fluorescence detection apparatus and an integrated PCR-CE microdevice, (b) Expanded view of the microfabricated PCR chamber, (c) Expanded cross-sectional view of the junction between the PCR and CE devices. The size of the epoxy filled gaps is exaggerated for viewing clarity. Reprinted with permission from Woolley etal. Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DMA Analysis Device. Reprinted with permission from [5]...

Figure 10.4 Schematics and photographs of the integrated PCR-CE microdevice, (a) Pictures of the integrated microfluidic device, (b) Pictures of glass chip (multiple PCR unit and multiple channel separation unit), (c) Schematics of the integrated microfluidic...

Current developments are not only aimed at integration but also at parallel genetic analysis as well. Parallel genetic analysis allows detection of different DNA samples simultaneously. A microfluidic device integrated with multichamber PCR and multichannel CE for parallel genetic analysis was developed (Figure 10.4) [56]. The microdevice consists of three functional units temperature control, multiple PCR (four chambers PCR), and multiple channel separation (four separation channels, each channel cormected to a PCR chamber). A separation channel is... 

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