Integrated fluorescence detector

Webster, J.R., Burns, M.A., Burke, D.T., Mastrangelo, C.H., Monolithic capillary electrophoresis device with integrated fluorescence detector Anal. Chem. 2001, 73(7), 1622-1626. 

Webster JR, Burns MA, Burke DT, Mastrangelo CH (2001) Monolithic Capillary Electrophoresis Device with Integrated Fluorescence Detector. Anal Chem 73 1622-1626... 

The liquid chromatograph Zarghi et al. used consisted of a Wellchrom K-1001 pump, Rheodyne 7125 injector, Eurochrom 2000 integrator, and K2600 fluorescence detector. The stationary phase was a Merck Chromolith Performance RP-18e column (100 x 4.6 mm). The mobile phase consisted of 0.01M dibasic sodium phosphate buffer and acetonitrile (60 40 v/v) adjusted to pH 3.5. The flow rate was 2 mL/min. The detector operated at an excitation wavelength of 240 nm and an emission wavelength of 340 nm. Letrozole served as the internal standard (IS). 

The HPLC gradient system consisted of two pumps for solvent delivery, an injector, a 120 x 4.6 mm Polyspher AA-NA column (Merck, 75°C), a pump for reagenf delivery, a mixing tee with reaction coil, and a fluorescence detector lined to an integrator (Waters). 

Often, treatment of samples with fluorescence labeling agent reacts with primary and secondary amines to give a fluorescent compound. This is especially important for detecting amino acids in protein hydrolyzates. Fluorescence detectors may also be integrated into a high performance liquid chromatographic (HPLC) system. 

HPLC system consisting of a buffer pump, preferably with degasser, autosampler with cooling system, fluorescence detector (excitation 384 nm, emission 516 nm) and an integration and data handling system. 

Abbreviations AOD, Acousto-optical deflection BCB, bisbenzyocyclobutadiene CCD, indirect contact conductivity detection CL, chemiluminescence ECD, electron capture detector FCS, fluorescence correlation spectroscopy FRET, fluorescence resonance energy transfer ICCD, integrated contact conductivity detection GMR, giant magnetoresistive LED-CFD, light emitting diode confocal fluorescence detector LIF, laser-induced fluorescence LOD, limit of detection MALDI, matrix-assisted laser desorption ionization PDMS, poly(dimethylsiloxane) PMMA, poly(methylmetha-crylate) SPR, surface plasmon resonance SVD, sinusoidal voltammetric detection TLS, thermal lens spectroscopy. 

SMSI (Montgomery County, MD www.s4ms.com/) is developing a fully implantable system that integrates fluorescence glucose-sensing chemistry, light source, detector,... 

From the integrated signals of the RI detector, which is concentration-sensitive, and the fluorescence detector, which is functional group-sensitive, the total amount of carbonyl or carboxyl groups can be calculated. 

What are the light source and detector used in the microchip with integrated fluorescent detection [692] (2 marks)... 

Adams, M.L., Enzelberger, M., Quake, S., Scherer, A., Microfluidic integration on detector arrays for absorption and fluorescence micro-spectrometers Sensors... 

BAWS The bio-aerosol warning sensor (BAWS) is an ultraviolet (UV) fluorescence detector using laser illumination. BAWS units are planned to be integrated into the joint biological point detection system (JBPDS) as a trigger for the presence of a 1- to 10-micron-sized biological particle. 

Li H-F, Lin J-M, Su R, Uchiyama K, Hobo T. A compactly integrated laser-induced fluorescence detector for microchip electrophoresis. Electrophoresis 2004 25 1907-15. 

Recently, a novel type of integrated optical detectors based on the interaction of radiation with a surface situated in a flowing stream was introduced [848]. Exploiting absorbance, reflectance, and fluorescence of visible and/or UV light as it changes due to chemical reactions taking place at or in close proximity of a surface surrounded by a flowing stream,... 

In this section we describe in more detaii the three key opticai components for integrated fluorescence detection (1) iight sources, (2) detectors, and (3) opticai fliters. We aiso present proof-of-principie studies from our group describing the use of these components in chemicai and bioiogicai anaiysis. 

Fig. 2 Schematic diagram of free jet apparatus showing the positions of the lasers and the fluorescence detector. On the right side of the diagram, the timing sequence is illustrated for the excimer and dye laser as well as for the integrator gate.

A detective system by integration of fluorescence detector based on a microavalanche photodiode into a PDMS microfluidic device [3,4]... 

Chabinyc et al. [2] succeeded the integration of fluorescence detector based on a microavalanche photodiode into a PDMS-based microfluidic device. Their detective system was reusable, and the microfluidic device was disposable. Elimination of the index matching problem which can be problematic in some micromachined devices, the elimination of the need for collection optics, and the inexpensiveness are the superiority of the design. They also addressed some further improvements for the performance of the device. 

The reduction of dimensions also reduces volumes that are accessible for the detector. Thus, detection principles that are related to the volume of the detector cell, as absorbance measurements, are not ideally suited for coupling to microsystems, whereas surface sensitive principles, such as electrochemical methods or optical methods utilizing the evanescent field of a waveguide, or methods that can be focused on a small amount of liquid, such as fluorescence, are better suited. That is why fluorescence detectors are most often combined with microsystems, with an increasing number of reports appearing on the integration of electrochemical detectors. 

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