Institute of Microbiology and Experimental Therapy

HERMANN BERG Academy of Sciences of the GDR, Central Institute of Microbiology and Experimental Therapy, Department of Biophysical Chemistry, DDR-69 Jena, GDR. 

CT-DNA Calf-thymus DNA was a kind gift of Dr.G.Luck (Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR) with a content of 0.28 % of protein, stabilized with chloroform. The stock solution was diluted to an OO26O 0.625 by means of a O.OOl M NaCl solution. 

This appendix has been added to the English edition of this book as a consequence of a critical recommendation by I.J. Dunn (Federal Technical University/ Lab of Technical Chem. Zurich). Most of the simulations were realized at Graz the University of Technology, Austria, or at ZIMET, Central Institute of Microbiology and Experimental Therapy, Jena, East Germany (Fig. 11.33 and 11.34). 

Comparison of proteinase regulation during growth and development was based on the study of Bacillus megaterium 27, kindly provided by Prof. Udo Taubeneck from the Institute of Microbiology and Experimental Therapy,... 

L. of culture suspension of Claviceps purpurea (Fr.), Tul. IMET PA 130 (obtained from the Jena Central Institute for Microbiology and Experimental Therapy, GDR) is stirred for 30 minutes with 8 kg bentonite. It is then filtered through a 10 mm layer of calcium sulfate dihydrate over a rotary vacuum cell filter the practically alkaloid-free filtrate is discarded. The recovered mycella-bentonite mixture is dried in a fluidized bed at an influx temperature of 90° C. until an exhaust temperature of 60° C. is achieved. This takes approximately 30 minutes. There is recovered 20-24 kg of mycella-bentonite mixture with a residual moisture content of from 3-5% this mixture contains 95-100% of the ergotoxin and 92-96% of the ergometrin contained in the culture suspension. 

Gutke, R. (1980). In UNEP/UNESCO/ICRO training course. Theoretical Basis of Kinetics of Growth, Metabolism and Product Formation of Microorganisms. Jena Science Academy of East Germany, Central Institute for Microbiology and Experimental Therapy (ZIMET), Vol. 1, pps. 30, and 112. 

Acknowledgment. Most of the computer simulations in Chaps. 5 and 6 were performed by the group of Prof. Dr. W. Knorre of ZIMET (Central Institute for Microbiology and Experimental Therapy, Biotechnology Division) at the Academy of Sciences of the German Democratic Republic in Jena (Prof. F. 

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