Institute of Brewing

There are currently a number of recommended methods of analysis for the determination of a-and 0-acids in hops by HPLC (e.g., Fig. 2). The methods recommended by the Institute of Brewing, the American Society of Brewing Chemists, and the European Brewery Convention are summarized in Table 1. The method recommended by the latter two is in fact the same and is thus considered to be an international method. There are some key differences worthy of note here. Both clearly rely on the use of phosphoric acid to suppress the ionization of the acidic compo-... 

Institute of Brewing American Society of Brewing Chemists (ASBC) European Brewery Convention... 

JR Hudson. Development of Brewing Analysis. London Institute of Brewing, 1960, pp 36-59. 

Institute of Brewing Recommended Methods. Hops and hop products 6.5. Alpha- and beta-acids in hop extracts by HPLC. 

Figure 10. Interferograph from a draining a/w suspended thin film showing the calculated change in film thickness with time. Reproduced from reference [20] with the permission of the Institute of Brewing.

The Institute of Brewing Analysis Committee (P. A. Martin, Chairman), J. Inst. Brew., 79 (1973) 289-293. 

Anderson, R. G., Kirsop, B. H. (1974) Control of volatile ester synthesis during fermentation of wort of high specific gravity. Journal of the Institute of Brewing, 80, 48-55. 

Ayrapaa, T. (1971) Biosynthetic formation of higher alcohols by yeast. Dependence on the nitrogen nutrient level of the medium. Journal of the Institute of Brewing, 77, 266-275. 

Erten, H., Campbell, L. (2001) The production of low-alcohol wines by aerobic yeasts. Journal of the Institute of Brewing, 107, 207-215. 

Nordstrom, K. (1964) Formation of ethyl acetate in fermentation with brewer s yeast. V. Effect of some vitamins and mineral nutrients. Journal of the Institute of Brewing, 70, 209-221. 

Rossi, 1., Bertuccioh, M. (1992) Influences of lipid additions on fatty acid composition of Saccharomyces cerevisiae and aroma characteristics of experimental wines. Journal of the Institute of Brewing, 98, 305-314. 

Vaudano, E., Moruno, E. G., Di Stefano, R. (2004) Modulation of geraniol metabolism during alcohol fermentation. Journal of the Institute of Brewing, 110, 213-219. 

S. Aiba and M. Nagatani Institute of Applied Microbiology, University of Tokyo, Tokyo, Japan Institute of Brewing,... 

Pilkington P.H., Margaritis A., Mensour N.A. and Russell I. Fundamentals of irmnobilized yeast cells for continuous beer fermentation A review. Journal of the Institute of Brewing 104 (1) (1998) 19-31. 

Branyik T., Vicente A. A., Dostalek P. and Teixeira J.A. A review of flavom formation in continuous beer fermentations. Journal of the Institute of Brewing 114 (1) (2008) 3-13. 

Moll M. Fermentation and maturation of beer with immobilised yeasts. Journal of the Institute of Brewing 112 (4) (2006) 346-346. 

Genisheva Z., Macedo S., Mussatto S.I., Teixeira J.A. and Oliveira J.M. Production of white wine by Saccharomyces cerevisiae immobilized on grape pomace. Journal of the Institute of Brewing 118 (2)... 

Institute of Brewing, Hops Advisory Committee (1970), J. Inst. Brewing, 76, 295. 

Institute of Brewing Analysis Committee (1948). /. Inst. Brewing, 54, 179. 

The major ions are the cations potassium, sodium, magnesium and calcium with the anions chloride, sulphate, nitrate and phosphate. The level of toxic metals may be limited by law. In Britain the level of arsenic (1959) and lead (1979) may not exceed 0-2 mg/kg (ppm) and the Food Standards Committee have recommended limits of 7-0 ppm for copper and 5 0 ppm for zinc in both wine and beer. The EBC, ASBC and the Institute of Brewing all describe methods for the estimation of iron and copper in beer. In addition the EBC gives methods for calcium, nickel, potassium, sodium and zinc the ASBC for calcium and phosphorous and the Institute of Brewing for arsenic, lead and zinc. 

Much of the sulphur dioxide present in beer is in a bound form, for example, as the acetaldehyde bisulphite compound. The classical (Monier-Williams) method for the estimation of sulphur dioxide, adopted by the Institute of Brewing, involves the removal of SO2 from acidified beer in a stream of carbon dioxide and nitrogen at 100 C. The gas is absorbed in hydrogen peroxide and the sulphuric acid formed titrated. In Britain sulphur dioxide is a permitted preservative in beers ( > 70 ppm), ciders, and wines ( > 150 ppm) and the Monier-Williams method is official. The EBC-ASBC adopt a more sensitive colorimetric method in which the colour restored to acid-decolourized rosaniline hydrochloride is measured. 

The Institute of Brewing use the EBC method for malts but have adopted a photometric method for worts and beers based on measurements at 530 nm. The photometer is previously calibrated with a standard potassium dichromate solution and the result is expressed directly in EBC units of colour [118]. 

Institute of Brewing (1977). Recommended Methods of Analysisj The Institute of Brewing, London. 

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