Immobilization insect cells

Wang MY, Kuo YY, Lee MS, Doong SR, Ho JY, Lee LH (2000), Self-assembly of the infectious bursal disease virus capsid protein, rVP2, expressed in insect cells and purification of immunogenic chimeric rVP2H particles by immobilized metal-ion affinity chromatography, Biotechnol. Bioeng. 67 104-111. 

Hu Y.C., Bentley W.E., Edwards G.H. and Vakharia V.N. 1999. Chimeric infectious bursal disease virus-like particles expressed in insect cells and purified by immobilized metal affinity chromatography, Biotechnol. Bioeng., 63, 721. 

Techniques of attachment, entrapment, and encapsulation are most widely used for cell immobilization with support materials, which are illustrated in Figure 7.1. These techniques can be applied to essentially all the viable or nonviable wholecell systems of potential interest microorganisms, plant cells, and mammalian and insect cells [2]. Although most of the principles associated with enzyme immobilization are directly applicable to cell immobilization, due to the complete difference in size and biochemical properties between enzymes the cells, the relative importance of these methods is considerably different [10]. 

Immobilized Cells and their Applications 223 Table 7.3 Recombinant proteins produced by immobilized mammalian and insect cells. 

Immobilized insect cells -Galactosidase Spodopterafrugiperda (Sf-9) Entrapped within reticulated [114,115]... 

Yamaji, H., Manabe, X, Kitaura, A., Izumoto, E. et al (2006) Efficient production of recombinant protein in immobilized insect cell culture using serum-free basal media after bac-ulovirus infection. Biochem. Eng. J., 28, 67 -72. 

Kondo, A. et al (2007) Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles. /. Biosci. Bioeng., 103,... 

Leaves pale and discolored tiny brown bumps on undersides and stems. Cause Scales. These immobile insects suck fluids from plant cells. They may excrete sticky honeydew onto nearby leaves. Isolate affected plants. Remove heavily infested leaves. Gently scrape insects... 

This paper describes a novel thermally reversible chitosan oxalate gel, a thermally irreversible chitosan gel, and a thermally irreversible chitin gel (N-acetylchitosan gel or regenerated chitin). An intramolecular conversion of the chitosan oxalate gel into the chitin gel via the chitosan gel is discussed. The chitin gel may play a role of the structural processing of crab and shrimp shells and insect cuticle. A sheet of transparent film has been prepared from the gel slice by air-drying, 2 and a sheet of non-transparent porous by freeze-drying. The gel may be usable as a digestible material for drug delivery systems and as media for the immobilization of enzymes and cells. 

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