INDEX Lettuce

So, these results showed a double effect of BOA on lettuce meristems an increasing significant delay in the cell cycle progression and a decrease in the mitotic index. Flow cytometry analysis showed a weak effect of BOA at cell cycle level in the step from G2 to M. BOA effect appeared to retard the cell cycle progression of treated-root meristems, which was very clear after 10 h BOA exposition (Fig. 12.5), and as an inhibition of the number of cells undergoing cell division. 

Mitotic index in root tip cells from lettuce meristems treated for 2-14 h with 1 mM BOA (treated meristems) or distilled water (control meristems) after 6 h HU synchronization. Mitotic cells were counted for each treatment every 2 h after HU release (From Sanchez-Moreiras, A. M. et al. 2001. Handbook of Plant Ecophysiology Techniques. Kluwer Academic Publishers, Dordrecht, The Netherlands, 81-95. With permission). 

We are grateful to Dr. Marina Horjales, Dr. Nieves Redondo, and Alfonso Blanco (Faculty of Sciences, University of Vigo) for their help in the optimization of cell cycle analysis and mitotic index technique applied to lettuce. The authors also want to thank Claudia Carcamo and Oliver Weiss for their continual and valuable help in these experiments. Financial support from Ministry of Science and Technology (Spanish Government) is also acknowledged. 

Key Word Index allelochemical activity, bioassay, continuous cropping, epicotyl, extraction, inhibitory, lettuce, life cycle, mass spectrometry, mono and bi-desmoside saponins, mungbeans, purification, radicle, soyasaponins, soil, stimulation, thin layer chromatography, Vigna radiatu L., Lactuca sativa L. 

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