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Inactivation structure

In this bromoaspirin-inactivated structure, Ser , which lies along the wall of the tunnel, is bromoacetylated, and a molecule of salicylate is also bound in the tunnel. Deep in the tunnel, at the far end, lies Tyr, a catalytically important residue. Heme-dependent peroxidase activity is implicated in the formation of a proposed Tyr radical, which is required for cyclooxygenase activity. Aspirin and other NSAIDs block the synthesis of prostaglandins by filling and blocking the tunnel, preventing the migration of arachidonic acid to Tyr in the active site at the back of the tunnel. [Pg.835]

Carbonyl reagents, such as semicarbazide and phenelzine (27), are inactivators of SSAO. In a strategy that includes two inactivating structural motifs (allylamine and hydrazine), a series of allyl hydrazines including the series 28a-c as well as the fluoroallyl analogue 29 were prepared. Compounds 28a-c were potent irreversible inhibitors of SSAO, and compounds 28a,c had particularly good selectivity with respect to MAO inhibition. The presence of the vinyl fluoride in 29 had little effect on potency but did result in a loss in selectivity [82]. [Pg.674]

Fig. 16.8. Model of inactivation of voltage-gated Na and K channels, a) Inactivation of the Na channel. On inactivation of the Na channel, the loop, which binds domain III and domain IV of the a-subunit, positions itself in the cytoplasmic entrance of the pore and closes it. The indicated hydrophobic amino acids of the connecting loop are involved in the inactivation, b) Inactivation of the K channel. The model assumes that a compact structural part of the C terminus of the P subunit is aligned in the pore and transiently closes it. The inactivating structural part is linked to the pore via a flexible structural element and contains a functionally important leucine residue and a lot of positive charges. According to CatteraU, (1995). Fig. 16.8. Model of inactivation of voltage-gated Na and K channels, a) Inactivation of the Na channel. On inactivation of the Na channel, the loop, which binds domain III and domain IV of the a-subunit, positions itself in the cytoplasmic entrance of the pore and closes it. The indicated hydrophobic amino acids of the connecting loop are involved in the inactivation, b) Inactivation of the K channel. The model assumes that a compact structural part of the C terminus of the P subunit is aligned in the pore and transiently closes it. The inactivating structural part is linked to the pore via a flexible structural element and contains a functionally important leucine residue and a lot of positive charges. According to CatteraU, (1995).
Silverman and Zieske have rationalized how a protein nucleophile other than flavin is involved in MAO inactivation reactions, and why different inactivator compounds specifically react with flavin, protein amino acids, or both (100). Hydrogen atom donation from a cysteine residue to the flavin semiquinone radical would produce a thiyl radical, which could then capture the primary or secondary alkyl radical generated on cyclopropyl ring opening from the amine radical cation of the inactivator. The hydrogen atom abstraction reaction between the flavin and active site amino acid may be an equilibrium process such that either species could be present at any turnover. Hence, a combination of steric constraints and proximity to either the flavin semiquinone radical or the thiol radical will determine the site of adduct formation for a particular inactivator structure. A two-dimensional representation is shown in Scheme 23 (compounds 40-42), which illustrates the proposed equilibrium between the flavin semiquinone radical and amino acid as well as the proposed intermediates for the inactivation of MAO by A-(l-methylcyclopropyl)benzylamine 40 (104), rrradical center relative to the particular protein radical is consistent with proposed site of attachment of inactivator to protein 40 is near the flavin radical, such that exclusive flavin attachment occurs, 41 is positioned closer to the amino... [Pg.353]

Ortiz de Montellano, P.R. and K.L. Kunze (1981). Cytochrome P-450 inactivation Structure of the prosthetic heme adduct with propyne. [Pg.236]

Zachariae U, Schneider R, Velisetty P et al (2008) The molecular mechanism of toxin-induced conformational changes in a potassium channel relation to C-type inactivation. Structure 16 747-754... [Pg.205]

Src tyrosine kinase contains both an SH2 and an SH3 domain linked to a tyrosine kinase unit with a structure similar to other protein kinases. The phosphorylated form of the kinase is inactivated by binding of a phosphoty-rosine in the C-terminal tail to its own SH2 domain. In addition the linker region between the SH2 domain and the kinase is bound in a polyproline II conformation to the SH3 domain. These interactions lock regions of the active site into a nonproductive conformation. Dephosphorylation or mutation of the C-terminal tyrosine abolishes this autoinactivation. [Pg.280]

Lysozyme from bacteriophage T4 is a 164 amino acid polypeptide chain that folds into two domains (Figure 17.3) There are no disulfide bridges the two cysteine residues in the amino acid sequence, Cys 54 and Cys 97, are far apart in the folded structure. The stability of both the wild-type and mutant proteins is expressed as the melting temperature, Tm, which is the temperature at which 50% of the enzyme is inactivated during reversible beat denat-uration. For the wild-type T4 lysozyme the Tm is 41.9 °C. [Pg.354]

Bacterial bioluminescence, 30-46 factors required, 31 general scheme, 32 in vivo luminescence, 41 luminescence reaction, 37, 38 Bacterial luciferase, 33-35, 343 assay, 39 cloning, 34 crystal structure, 34 extraction and purification, 34 inactivation, 34, 35 molecular weight, 34 properties, 34 storage, 35 subunits, 34... [Pg.456]

The reactivation of enzymes (after their partial inactivation in an acid medium) upon passing into a medium of pH 8 is also of great importance for oral use (Fig. 25). Enzymes immobilized in crosslinked polyelectrolytes are characterized by a structural memory even after considerable inactivation. Under changed conditions, this leads to a considerable or almost complete reactivation of the enzyme, whereas in the reactivation of a free enzyme in solution under similar conditions the enzymatic activity is restored on a lower level. [Pg.35]

Adaptor Proteins. Figure 1 Adaptor protein domains. A scheme of the domain structures of some well-characterized adaptor proteins is shown. Descriptions of domain characteristics are in main text except C2, binds to phospholipids GTPase activating protein (GAP) domain, inactivates small GTPases such as Ras Hect domain, enzymatic domain of ubiquitin ligases and GUK domain, guanylate kinase domain. For clarity, not all domains contained within these proteins are shown. [Pg.15]

In order to accomplish these diverse physiological tasks described above, nature has created at least five different types of Ca2+ channels. These are termed L-, N-, P/Q-, R-, and T-type. Although they are all structurally similar (Fig. 1) they differ with respect to their biophysical properties. Some of them need only weak depolarizations to open and inactivate fast (e.g., T-type Ca2+ channels), whereas others require strong depolarizations and inactivate more slowly (e.g. P- or L-type Ca2+ channels). Channel types also differ with respect to their sensitivity to drugs. This selectivity is exploited for pharmacotherapy. [Pg.296]

Summarizing the fibrinolytic therapy, it should be emphasized that efficient treatment needs urgent application of plasminogen activator (within a few hours) to prevent the formation of crosslinks in the fibrin structure (Fig. 2) and to find the localization of thrombus to emerge plasmin on the surface of fibrin to prevent rapid inactivation of the enzyme by the inhibitor system of fibrinolysis (Fig. 3). [Pg.506]

P-Lactamases are enzymes that hydrolyze the P-lactam ring of P-lactamantibiotics (penicillins, cephalosporins, monobactams and carbapenems). They are the most common cause of P-lactam resistance. Most enzymes use a serine residue in the active site that attacks the P-lactam-amid carbonyl group. The covalently formed acylester is then hydrolyzed to reactivate the P-lacta-mase and liberates the inactivated antibiotic. Metallo P-lactamases use Zn(II) bound water for hydrolysis of the P-lactam bond. P-Lactamases constitute a heterogeneous group of enzymes with differences in molecular structures, in substrate preferences and in the genetic localizations of the encoding gene (Table 1). [Pg.771]

The antimetabolites interfere with various metabolic functions of cells, thereby disrupting normal cell functions. They inactivate enzymes or alter the structure of DNA, changing the DNA s ability to replicate These drag are most effective in the treatment of rapidly dividing neoplastic cells. Examples of the antimetabolites include methotrexate and fluorouracil (Adrucil). [Pg.592]

Bock, P.E. Frieden, C. (1976). Phosphofructokinase I. Mechanism ofpH-dependent inactivation and reactivation of the rabbit muscle enzyme. II. Role of ligands in pH-dependent structural changes of the rabbit muscle enzyme. J. Biol. Chem. 251, 5630-5643. [Pg.276]

Figure 5.21 Structure of tazobactam. Reprinted from Biochim. Biophys. Acta, 1547, Bonomo, R. A., Liu, J., Chen, Y., Ng, L., Hujer, A. M. and Anderson, V. E., Inactivation of CMY-2 /3-lactamase by tazobactam initial mass spectroscopic characterization , 196-205, Copyright (2001), with permission from Elsevier Science. Figure 5.21 Structure of tazobactam. Reprinted from Biochim. Biophys. Acta, 1547, Bonomo, R. A., Liu, J., Chen, Y., Ng, L., Hujer, A. M. and Anderson, V. E., Inactivation of CMY-2 /3-lactamase by tazobactam initial mass spectroscopic characterization , 196-205, Copyright (2001), with permission from Elsevier Science.

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See also in sourсe #XX -- [ Pg.211 , Pg.212 ]

See also in sourсe #XX -- [ Pg.211 , Pg.212 ]




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Inactivation structural basis

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